Anti-PIK3R2 Polyclonal Antibody is a Rabbit antibody targeting PIK3R2. Anti-PIK3R2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Dog,Cow)

1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (P85B) Polyclonal Antibody, Unconjugated (TMAB-11450) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by microwave in sodium citratebuffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RTfor 30 min; Antibody incubation with (PIK3R2) Polyclonal/MonoclonalAntibody, Unconjugated (TMAB-11450) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
3. Sample: Colon carcinoma (Human) Lysate at 40 μg
Primary: Anti-PI 3 Kinase p85 beta (TMAB-11450) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution
Predicted band size: 82 kD
Observed band size: 85 kD
4. Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by microwave in sodium citratebuffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30 min; Antibody incubation with (PIK3R2) Polyclonal/MonoclonalAntibody, Unconjugated (TMAB-11450) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PIK3R2) Polyclonal Antibody, Unconjugated (TMAB-11450) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
6. Sample:
Lane 1: Mouse Muscle tissue lysates
Lane 2: Rat Muscle tissue lysates
Primary: Anti-PIK3R2 (TMAB-11450) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 82 kDa
Observed band size: 90 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human PI 3 Kinase p85 beta

Human

PIK3R2

Phosphatidylinositol 3-kinase regulatory subunit beta

Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.