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Anti-PIK3CA Antibody (9D297)
还可以产品编号 TMAH-00978
Anti-PIK3CA Antibody (9D297) 是一种抗体，靶向 PIK3CA。Anti-PIK3CA Antibody (9D297) 可用于 ELISA, WB, IF。
Anti-PIK3CA Antibody (9D297)
Anti-PIK3CA Antibody (9D297)
还可以产品编号 TMAH-00978
Anti-PIK3CA Antibody (9D297) 是一种抗体，靶向 PIK3CA。Anti-PIK3CA Antibody (9D297) 可用于 ELISA, WB, IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,300 | 5日内发货 | |
| 100 μL | ¥ 2,190 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-PIK3CA Antibody (9D297) is an antibody targeting PIK3CA. Anti-PIK3CA Antibody (9D297) can be used in ELISA, WB, IF. |
| Ig Type | Rabbit IgG |
| 克隆号 | 9D297 |
| 交叉反应 | Human, Mouse |
| 验证活性 | 1. Western Blot -Positive WB detected in: Jurkat whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate -All lanes: PIK3CA antibody at 1:1500 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 125 kDa -Observed band size: 110 kDa 2. Immunofluorescence staining of Hela Cells with TMAH-00978 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| 应用 | ELISA, WB, IF |
| 推荐剂量 | WB:1:500-1:5000; IF:1:20-1:200. |
| 抗体种类 | Monoclonal |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) and its phosphorylated derivatives at position 3 of the inositol ring to produce 3-phosphoinositides. Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. In addition to its lipid kinase activity, it displays a serine-protein kinase activity that results in the autophosphorylation of the p85alpha regulatory subunit as well as phosphorylation of other proteins such as 4EBP1, H-Ras, the IL-3 beta c receptor and possibly others. Plays a role in the positive regulation of phagocytosis and pinocytosis. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human PI 3 Kinase catalytic subunit alpha |
| 抗原种属 | Human |
| 基因ID | 5290 |
| Uniprot ID | |
| 研究领域 | Cancer, Immunology, Signal transduction |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
关键词
Serine/threonine protein kinase PIK3CAPtdIns-3-kinase subunit αPtdIns-3-kinase subunit alphaPtdIns-3-kinase subunit aPIK3CAPI3K-αPI3KαPI3-kinase subunit αPI3-kinase subunit alphaPI3-kinase subunit aPI3K-alphaPI3KalphaPI3K-aPI3KaPhosphoinositide-3-kinase catalytic α polypeptidePhosphoinositide-3-kinase catalytic alpha polypeptidePhosphoinositide-3-kinase catalytic a polypeptidePhosphoinositide 3-kinase αPhosphoinositide 3-kinase alphaPhosphoinositide 3-kinase aPhosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit α isoformPhosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoformPhosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit a isoformPhosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit α (PtdIns-3-kinase subunit p110-α;p110α)Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha (PtdIns-3-kinase subunit p110-alpha;p110alpha)Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit a (PtdIns-3-kinase subunit p110-a;p110a)p110αp110alphap110ap110 αp110 alphap110 a5-bisphosphate 3-kinase catalytic subunit α isoform5-bisphosphate 3-kinase catalytic subunit alpha isoform5-bisphosphate 3-kinase catalytic subunit a isoform
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