Anti-PI3Kβ Polyclonal Antibody is a Rabbit antibody targeting PI3Kβ. Anti-PI3Kβ Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG

Human,Mouse,Rat

1. 4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (PI3-kinase p110 subunit beta) polyclonal Antibody, unconjugated (TMAB-01535) 1:200, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.
2. Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 Kinase p110 beta Polyclonal Antibody, Unconjugated (TMAB-01535) at 1:100 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

WB: 1:500-1000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-1:200; IF: 1:50-200

Polyclonal

KLH conjugated synthetic peptide: human PI3 Kinase p110 beta

Human

Kinases/Phosphatases,TLR Signaling,Other Kinases,Lipid Kinases