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Anti-PI3 kinase p150 Antibody (7M212)

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货号 TMAB-11429

Anti-PI3 kinase p150 Antibody (7M212) 是一种 Rabbit 抗体，靶向 PI3 kinase p150。Anti-PI3 kinase p150 Antibody (7M212) 可用于 WB,IHC-P,IHC-Fr,IF,FCM,ICC/IF。

Anti-PI3 kinase p150 Antibody (7M212)

Anti-PI3 kinase p150 Antibody (7M212)

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Rating icon 还可以
货号 TMAB-11429

Anti-PI3 kinase p150 Antibody (7M212) 是一种 Rabbit 抗体，靶向 PI3 kinase p150。Anti-PI3 kinase p150 Antibody (7M212) 可用于 WB,IHC-P,IHC-Fr,IF,FCM,ICC/IF。

规格价格库存数量
25 μL
¥ 780
5日内发货
50 μL
¥ 1,390
5日内发货
100 μL
¥ 2,485
5日内发货
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产品介绍

生物活性
产品描述
Anti-PI3 kinase p150 Antibody (7M212) is a Rabbit antibody targeting PI3 kinase p150. Anti-PI3 kinase p150 Antibody (7M212) can be used in WB,IHC-P,IHC-Fr,IF,FCM,ICC/IF.
Ig Type
IgG
克隆号
7M212
反应种属
Human,Mouse,Rat
验证活性
1. Paraformaldehyde-fixed, paraffin embedded Mouse Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 kinase p150Monoclonal Antibody, Unconjugated (TMAB-11429) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 kinase p150Monoclonal Antibody, Unconjugated (TMAB-11429) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 kinase p150Monoclonal Antibody, Unconjugated (TMAB-11429) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 kinase p150Monoclonal Antibody, Unconjugated (TMAB-11429) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PI3 kinase p150Monoclonal Antibody, Unconjugated (TMAB-11429) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
6. 25 μg total protein per lane of various lysates (see on figure) probed with PI3 kinase p150 monoclonal antibody, unconjugated (TMAB-11429) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
应用WBIHC-PIHC-FrIFFCMICC/IF
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1:50-100; ICC/IF: 1:50-200
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式Protein A purified
性状Liquid
缓冲液10mM phosphate buffered saline, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
浓度1mg/ml
研究背景Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2.
抗原信息
免疫原
A synthesized peptide: human PI3 kinase p150
抗原种属
Human
基因名称
PIK3R4
基因ID
30849
蛋白名称
Phosphoinositide 3-kinase regulatory subunit 4
Uniprot ID
Q99570
化学信息
分子量Theoretical: 153 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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