Anti-Phospho-UBF1(Ser484) Antibody (2Z953) is a Rabbit antibody targeting Phospho-UBF1(Ser484). Anti-Phospho-UBF1(Ser484) Antibody (2Z953) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

2Z953

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-UBF1 (Ser484) Monoclonal Antibody, Unconjugated (TMAB-11408) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-UBF1 (Ser484) Monoclonal Antibody, Unconjugated (TMAB-11408) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-UBF1 (Ser484) Monoclonal Antibody, Unconjugated (TMAB-11408) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-UBF1 (Ser484) Monoclonal Antibody, Unconjugated (TMAB-11408) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-UBF1 (Ser484) Monoclonal Antibody, Unconjugated (TMAB-11408) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-UBF1 (Ser484) Monoclonal Antibody, Unconjugated (TMAB-11408) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-UBF1 (Ser484) Monoclonal Antibody, Unconjugated (TMAB-11408) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-UBF1 (Ser484) Monoclonal Antibody, Unconjugated (TMAB-11408) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-UBF1 (Ser484) Monoclonal Antibody, Unconjugated (TMAB-11408) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
10. Hela (H) cells were treated with or without Nocodazole, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-UBF1 (Ser484) monoclonal antibody, unconjugated (TMAB-11408) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
11. Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-UBF1 (Ser484) Monoclonal Antibody, Unconjugated (TMAB-11408) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Ratbbit IgG antibody (Red), DAPI (blue) was used to stain the cell nuclei.
12. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-UBF1 (Ser484) Monoclonal Antibody, Unconjugated (TMAB-11408) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Ratbbit IgG antibody (Red), DAPI (blue) was used to stain the cell nuclei.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

A synthesized peptide: human UBTF around the phosphorylation site of S484

Human

UBTF

Nucleolar transcription factor 1

Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.