Anti-Phospho-TORC2 (Ser171) Polyclonal Antibody is a Rabbit antibody targeting Phospho-TORC2 (Ser171). Anti-Phospho-TORC2 (Ser171) Polyclonal Antibody can be used in FCM,WB.

IgG

Human,Mouse (predicted:Rat,Dog,Pig)

1. Sample:
Lane 1: Kidney (Mouse) Lysate at 40 μg
Lane 2: Spleen (Mouse) Lysate at 40 μg
Primary: Anti-Phospho-TORC2 (Ser171) (TMAB-01524) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 90 kDa
Observed band size: 95 kDa
2. Blank control: Jurkat.
Primary Antibody (green line): Rabbit Anti-Phospho-TORC2 (Ser171) antibody (TMAB-01524)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Blank control: A431. Primary Antibody (green line): Rabbit Anti-Phospho-TORC2 (Ser171) antibody (TMAB-01524) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; FCM: 1ug/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human CREB regulated transcription coactivator 2 around the phosphorylation site of Ser171

Human

CRTC2

CREB-regulated transcription coactivator 2

Co-activators/co-repressors,Energy Metabolism,Cancer,Metabolism,Energy Metabolism,Co-activators/co-repressors

Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).