Anti-Phospho-Serine/Threonine Polyclonal Antibody is a Rabbit antibody targeting Phospho-Serine/Threonine. Anti-Phospho-Serine/Threonine Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF,ELISA.

IgG

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phosphoserine,threonine) Polyclonal Antibody, Unconjugated (TMAB-01491) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phosphoserine,threonine) Polyclonal Antibody, Unconjugated (TMAB-01491) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phosphoserine,threonine) Polyclonal Antibody, Unconjugated (TMAB-01491) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000

Polyclonal

KLH conjugated synthesised phosphopeptide contain Phosphoserine and Phosphothreonine

Amino acid metabolism,Phosphatases,Amino Acids,Tyrosine Phosphatases