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Anti-Phospho-S6 Ribosomal Protein (Ser240, 244) Antibody (9Q516)
还可以产品编号 TMAY-01576
Anti-Phospho-S6 Ribosomal Protein (Ser240, 244) Antibody (9Q516) 是一种 Rabbit 抗体，靶向 Phospho-S6 Ribosomal Protein (Ser240, 244)。Anti-Phospho-S6 Ribosomal Protein (Ser240, 244) Antibody (9Q516) 可用于 WB,IHC-P。
Anti-Phospho-S6 Ribosomal Protein (Ser240, 244) Antibody (9Q516)
Anti-Phospho-S6 Ribosomal Protein (Ser240, 244) Antibody (9Q516)
还可以产品编号 TMAY-01576
Anti-Phospho-S6 Ribosomal Protein (Ser240, 244) Antibody (9Q516) 是一种 Rabbit 抗体，靶向 Phospho-S6 Ribosomal Protein (Ser240, 244)。Anti-Phospho-S6 Ribosomal Protein (Ser240, 244) Antibody (9Q516) 可用于 WB,IHC-P。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Phospho-S6 Ribosomal Protein (Ser240, 244) Antibody (9Q516) is a Rabbit antibody targeting Phospho-S6 Ribosomal Protein (Ser240, 244). Anti-Phospho-S6 Ribosomal Protein (Ser240, 244) Antibody (9Q516) can be used in WB,IHC-P. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 9Q516 |
| 交叉反应 | Human; Species predicted to react based on 100% sequence homology: Mouse |
| 验证活性 | 1. Western blot analysis of extracts from serum-starved HEK293, untreated (-) or treated with 20% FBS, using Phospho-S6 Ribosomal Protein (Ser240, 244) Antibody, Rabbit MAb (upper), or Anti-RPS6 Antibody, Rabbit Polyclonal (lower) at 1:1000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human carcinoma of sigmoid tissue,untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-S6 Ribosomal Protein (Ser240/244) Antibody, Rabbit Monoclonal at 1:2000 dilution. 3. Western blot analysis of extracts from serum-starved HEK293, treated with 20% FBS (30 min), using Phospho-S6 Ribosomal Protein (Ser240, 244) Antibody, Rabbit MAb at dilution of 1:1000, 1:5000 and 1:25000. (Validation Experiment) 4. Western blot analysis of extracts from serum-starved HEK293, untreated (line A); treated with FBS(20%, 30min) (line B); treated with FBS and λ-phosphatase (line C) using Phospho-S6 Ribosomal Protein (Ser240, 244) rabbit monoclonal Antibody at 1:5000 dilution. (Validation Experiment) 5. Western blot analysis of extracts from serum-starved , untreated (line A); treated with FBS(20%, 30min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-S6 Ribosomal Protein (Ser240, 244) rabbit monoclonal Antibody at 1:5000 dilution. (Validation Experiment) |
| 应用 | WB,IHC-P |
| 推荐剂量 | WB: 1:1000-1:5000; IHC-P: 1:1000-1:5000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | The antibody is produced by immunizing rabbits with a synthetic phosphopeptide corresponding to residues around (Ser240, 244) of Human Phospho-S6 Ribosomal Protein. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| 研究背景 | Ribosomal protein S6 (rpS6), a component of the 40S ribosomal subunit, is involved in multiple cellular bioactivities. RPS6 is a downstream substrate of PI3K/Akt/mTOR/ kinase and MAPK kinase pathways and its dephosphorylation is also triggered by DNA double-strand breaks. The ribosomal protein S6 (RPS6) is a downstream component of the signaling mediated by the target of rapamycin (TOR) kinase that acts as a central regulator of the key metabolic processes, such as protein translation and ribosome biogenesis, in response to various environmental cues. mTORC1/rpS6 signaling complex promoted Sertoli blood-testis barrier (BTB) remodeling by perturbing Sertoli cell-cell adhesion site known as the basal ectoplasmic specialization (ES). mTORC1/rpS6 complex also promoted disruption of spermatid adhesion at the Sertoli-spermatid interface called the apical ES. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide corresponding to residues around (Ser240, 244) of Human Phospho-S6 Ribosomal Protein. |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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