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Anti-Phospho-S6 Ribosomal Protein (Ser235, 236) Antibody (1E580)
还可以产品编号 TMAY-01581
Anti-Phospho-S6 Ribosomal Protein (Ser235, 236) Antibody (1E580) 是一种 Rabbit 抗体，靶向 Phospho-S6 Ribosomal Protein (Ser235, 236)。Anti-Phospho-S6 Ribosomal Protein (Ser235, 236) Antibody (1E580) 可用于 WB。
Anti-Phospho-S6 Ribosomal Protein (Ser235, 236) Antibody (1E580)
Anti-Phospho-S6 Ribosomal Protein (Ser235, 236) Antibody (1E580)
还可以产品编号 TMAY-01581
Anti-Phospho-S6 Ribosomal Protein (Ser235, 236) Antibody (1E580) 是一种 Rabbit 抗体，靶向 Phospho-S6 Ribosomal Protein (Ser235, 236)。Anti-Phospho-S6 Ribosomal Protein (Ser235, 236) Antibody (1E580) 可用于 WB。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Phospho-S6 Ribosomal Protein (Ser235, 236) Antibody (1E580) is a Rabbit antibody targeting Phospho-S6 Ribosomal Protein (Ser235, 236). Anti-Phospho-S6 Ribosomal Protein (Ser235, 236) Antibody (1E580) can be used in WB. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 1E580 |
| 交叉反应 | Human; Species predicted to react based on 100% sequence homology: mouse |
| 验证活性 | 1. Western blot analysis of extracts from serum-starved Hek293, untreated (-) or treated with IGF1 (100ng/ml, 15min; +), using Phospho-S6 Ribosomal Protein (Ser235, 236) rabbit Monoclonal Antibody at 1:1000 dilution (upper) or anti-S6 Ribosomal Protein antibody (lower) at 1:1000 dilution. 2. Western blot analysis of extracts from serum-starved HEK293, treated with IGF1 (100 ng/mL, 15min), without peptide (line A) or antigen-specific phosphopeptide (line B) or antigen-specific peptide (line C) using Phospho-S6 Ribosomal (Ser235, 236) rabbit monoclonal Antibody at 1:1000 dilution. (Validation Experiment) 3. Western blot analysis of extracts from serum-starved HEK293, treated with IGF1 (100ng/mL, 15min) (line A); treated with IGF1 and λ-phosphatase (line B) using Phospho-S6 Ribosomal (Ser235, 236) rabbit monoclonal Antibody at 1:1000 dilution. (Validation Experiment) |
| 应用 | WB |
| 推荐剂量 | WB: 1:1000-1:5000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to residues around (Ser235, 236) of Human Phospho-S6 Ribosomal Protein. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| 研究背景 | Ribosomal protein S6 (rpS6), a component of the 40S ribosomal subunit, is involved in multiple cellular bioactivities. RPS6 is a downstream substrate of PI3K/Akt/mTOR/ kinase and MAPK kinase pathways and its dephosphorylation is also triggered by DNA double-strand breaks. The ribosomal protein S6 (RPS6) is a downstream component of the signaling mediated by the target of rapamycin (TOR) kinase that acts as a central regulator of the key metabolic processes, such as protein translation and ribosome biogenesis, in response to various environmental cues. mTORC1/rpS6 signaling complex promoted Sertoli blood-testis barrier (BTB) remodeling by perturbing Sertoli cell-cell adhesion site known as the basal ectoplasmic specialization (ES). mTORC1/rpS6 complex also promoted disruption of spermatid adhesion at the Sertoli-spermatid interface called the apical ES. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide corresponding to residues around (Ser235, 236) of Human Phospho-S6 Ribosomal Protein |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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