Anti-Phospho-PTPN6 (Ser591) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PTPN6 (Ser591). Anti-Phospho-PTPN6 (Ser591) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Rat (predicted:Mouse,Dog,Pig,Horse)

1. Paraformaldehyde-fixed, paraffin embedded (rat spleen tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PTPN6 (Ser591)) Polyclonal Antibody, Unconjugated (TMAB-11198) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample:
A549 Cell (Human) Lysate at 40 μg
MCF-7 Cell (Human) Lysate at 40 μg
Primary: Anti-phospho-PTPN6 (Ser591) (TMAB-11198) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD
3. Blank control: Hela Cells (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-phospho-PTPN6 (Ser591) antibody (TMAB-11198), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human PTPN6 around the phosphorylation site of Ser591

Human

PTPN6

Tyrosine-protein phosphatase non-receptor type 6

Modulates signaling by tyrosine phosphorylated cellcsurface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.