Anti-Phospho-PLK1 (Ser137) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-PLK1 (Ser137). Anti-Phospho-PLK1 (Ser137) Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Rat (predicted:Mouse,Chicken,Dog,Pig,Cow,Rabbit)

1. Paraformaldehyde-fixed, paraffin embedded (rat colon tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PLK1 (Ser137)) Polyclonal Antibody, Unconjugated (TMAB-11174) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample:
Lane 1: Human Jurkat cell lysates
Lane 2: Human A549 cell lysates
Lane 3: Human HeLa cell lysates
Lane 4: Human K562 cell lysates
Lane 5: Human U-2 OS cell lysates
Lane 6: Human HepG2 cell lysates
Lane 7: Human MDA-MB-231 cell lysates
Primary: Anti-Phospho-PLK1 (Ser137) (TMAB-11174) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 60 kDa
3. Blank control (black line): Hela.
Primary Antibody (green line): Rabbit Anti-Phospho-PLK1 (Ser137) antibody (TMAB-11174)
Dilution: 2 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2μg/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human PLK1 around the phosphorylation site of Ser137

Human

plk1

Serine/threonine-protein kinase PLK1

Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS.