Anti-Phospho-Paxillin (Tyr88) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Paxillin (Tyr88). Anti-Phospho-Paxillin (Tyr88) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat

1. Sample: 293T (Human) Cell Lysate at 30 μg
Primary: Anti-Phospho-Paxillin (Tyr88) (TMAB-11119) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 68 kD
2. Sample: Embryo (Mouse) Lysate at 40 μg
Primary: Anti-Phospho-Paxillin (Tyr88) (TMAB-11119) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 68 kD
3. Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (P-Paxillin (Tyr88)) Polyclonal Antibody, Unconjugated (TMAB-11119) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (mouse pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (P-Paxillin (Tyr88)) Polyclonal Antibody, Unconjugated (TMAB-11119) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (Rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-Paxillin (Tyr88)) Polyclonal Antibody, Unconjugated (TMAB-11119) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (Rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-Paxillin (Tyr88)) Polyclonal Antibody, Unconjugated (TMAB-11119) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-Paxillin (Tyr88)) Polyclonal Antibody, Unconjugated (TMAB-11119) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human Paxillin around the phosphorylation site of Tyr88

Human

PXN

Paxillin

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).