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Anti-Phospho-p38 MAPK (Thr180, Tyr182) Antibody (2X628)
还可以产品编号 TMAY-01511
Anti-Phospho-p38 MAPK (Thr180, Tyr182) Antibody (2X628) 是一种 Rabbit 抗体，靶向 Phospho-p38 MAPK (Thr180, Tyr182)。Anti-Phospho-p38 MAPK (Thr180, Tyr182) Antibody (2X628) 可用于 WB,IHC-P,ICC/IF。
Anti-Phospho-p38 MAPK (Thr180, Tyr182) Antibody (2X628)
Anti-Phospho-p38 MAPK (Thr180, Tyr182) Antibody (2X628)
还可以产品编号 TMAY-01511
Anti-Phospho-p38 MAPK (Thr180, Tyr182) Antibody (2X628) 是一种 Rabbit 抗体，靶向 Phospho-p38 MAPK (Thr180, Tyr182)。Anti-Phospho-p38 MAPK (Thr180, Tyr182) Antibody (2X628) 可用于 WB,IHC-P,ICC/IF。
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| 产品描述 | Anti-Phospho-p38 MAPK (Thr180, Tyr182) Antibody (2X628) is a Rabbit antibody targeting Phospho-p38 MAPK (Thr180, Tyr182). Anti-Phospho-p38 MAPK (Thr180, Tyr182) Antibody (2X628) can be used in WB,IHC-P,ICC/IF. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 2X628 |
| 交叉反应 | Human; Predicted to React with:Phospho-p38 MAPK (Thr180, Tyr182) |
| 验证活性 | 1. Immunohistochemical analysis of paraffin-embedded human carcinoma of sigmoid tissue,untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-p38 MAPK (Thr180-Tyr182) Antibody, Rabbit Monoclonal at 1:500 dilution. 2. Immunofluorescence staining of Phospho-p38 MAPK (Thr180, Tyr182) in serum-starved NIH-3T3 cells, untreated (left), treated with UV (30mins) (middle) or treated with UV (30mins) and lambda phosphatase (right). Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Mouse Phospho-p38 MAPK (Thr180, Tyr182) monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was mainly localized to Nucleus. 3. Western blot analysis of extracts from serum-starved NIH-3T3, untreated(line A) or treated with UV (30min; +)(line B), using Phospho-p38 MAPK (Thr180, Tyr182) rabbit monoclonal Antibody at 1:1000 dilution (upper) or Anti-Actin Antibody, Chimeric Rabbit Monoclonal at 1:50000 dilution (lower). 4. Western blot analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with UV (30min; +) (line B); treated with UV and λ-phosphatase (line C) using Phospho-p38 MAPK (Thr180, Tyr182) rabbit monoclonal Antibody at 1:1000 dilution. (Validation Experiment) 5. Western blot analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with UV (30min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-p38 MAPK (Thr180, Tyr182) rabbit monoclonal Antibody at 1:1000 dilution. (Validation Experiment) |
| 应用 | WB,IHC-P,ICC/IF |
| 推荐剂量 | WB: 1:1000-1:10000; IHC-P: 1:200-1:1000; ICC-IF: 1:20-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around Thr180, Tyr182 of human Phospho-p38 MAPK. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| 研究背景 | MAPK14 contains 1 protein kinase domain and belongs to the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. MAPK14 can be detected in the brain, heart, placenta, pancreas, and skeletal muscle and it is expressed to a lesser extent in the lung, liver, and kidney. MAPK14 is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with MAPK14. The substrates of p38 alpha include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of p38 alpha in stress-related transcription and cell cycle regulation, as well as in genotoxic stress response. In response to activation by environmental stress, pro-inflammatory cytokines, and lipopolysaccharide, MAPK14 phosphorylates some transcription factors, such as ELK1 and ATF2, and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. MAPK14 plays a critical role in the production of some cytokines, for example, IL-6. It may play a role in the stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic phosphopeptide corresponding to residues around Thr180, Tyr182 of human Phospho-p38 MAPK. |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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