Anti-Phospho-MEK6 (Ser202) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MEK6 (Ser202). Anti-Phospho-MEK6 (Ser202) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human)

1. Tissue/cell: Rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-MAPKK6 Polyclonal Antibody, Unconjugated (TMAB-10993) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample: Muscle (Mouse) Lysate at 40 μg
Primary: Anti-Phospho-MEK6 (Ser202) (TMAB-10993) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 38 kD
3. Sample:
Lane 1: Mouse Large intestine tissue lysates
Lane 2: Mouse Heart tissue lysates
Lane 3: Mouse Muscle tissue lysates
Primary: Anti-Phospho-MEK6 (Ser202) (TMAB-10993) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 42 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthesised phosphopeptide: human MEK6 around the phosphorylation site of Ser202

Human

MAP2K6

Dual specificity mitogen-activated protein kinase kinase 6

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.