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Anti-Phospho-MEF-2 (Thr312) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-MEF-2 (Thr312)。Anti-Phospho-MEF-2 (Thr312) Polyclonal Antibody 可用于 WB,IHC,IF。
Anti-Phospho-MEF-2 (Thr312) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-MEF-2 (Thr312)。Anti-Phospho-MEF-2 (Thr312) Polyclonal Antibody 可用于 WB,IHC,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,490 | 5日内发货 | |
100 μL | ¥ 2,245 | 5日内发货 |
产品描述 | Anti-Phospho-MEF-2 (Thr312) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MEF-2 (Thr312). Anti-Phospho-MEF-2 (Thr312) Polyclonal Antibody can be used in WB,IHC,IF. |
别名 | p-MEF-2 (Thr312), p-MEF-2 (T312), MEF-2 (p-Thr312), MEF-2 (p-T312) |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEF2A (Phospho-Thr312) Antibody TMAC-02519 (left) or the same antibody preincubated with blocking peptide (right). 2. Immunofluorescence staining of methanol-fixed Hela cells using MEF2A (Phospho-Thr312) Antibody TMAC-02519. 3. Western blot analysis of extracts from C2C12 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using MEF2A (Phospho-Thr312) Antibody TMAC-02519. |
应用 | |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
构建方式 | Polyclonal Antibody |
纯化方式 | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
性状 | Liquid |
缓冲液 | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including myoD (MIM 159970), myogenin (MIM 159980), MYF5 (MIM 159990), and MRF4 (MIM 159991) are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF2) family. Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes. The MEF2 genes are members of the MADS gene family (named for the yeast mating type-specific transcription factor MCM1, the plant homeotic genes 'agamous' and 'deficiens' and the human serum response factor SRF (MIM 600589)), a family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | Peptide sequence around phosphorylation site of threonine 312 (L-A-T(p)-P-V) derived from Human MEF2A |
抗原种属 | human |
Uniprot ID |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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