Anti-Phospho-MCU (S92) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MCU (S92). Anti-Phospho-MCU (S92) Polyclonal Antibody can be used in WB,IHC,IF.

IgG

Human,Mouse,Rat

1. All lanes: Phospho-MCU (S92) Rabbit mAb at 1/1k dilution, Lane 1: MCF7 whole cell lysates, Lane 2 : 293T whole cell lysates, Lane 3: A549 whole cell lysates, Lane 4 : Rat Liver lysates, Lysates/proteins at 20 μg per lane. Secondary All lanes: Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution, Predicted band size: 40 kDa, Observed band size: 32 kDa, Exposure time: 8 seconds.
2. All lanes: Phospho-MCU (S92) Rabbit mAb at 1/1k dilution, Lane 1: 3T3 whole cell lysates, Lane 2: 293T whole cell lysates, Lane 3: 293T treated with Lambda Protein Phosphatase for 30min whole cell lysates, Lane 4: MCF7 whole cell lysates, Lane 5: MCF7 treated with Lambda Protein Phosphatase for 30min whole cell lysates, Lysates/proteins at 20 μg per lane. Secondary All lanes: Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution, Predicted band size: 40 kDa, Observed band size: 32 kDa, Exposure time: 10 seconds.
3. Formalin-fixed, paraffin-embedded human lung carcinom tissue stained for Phospho-MCU (S92) using 14297 at 1/100 dilution in immunohistochemical analysis.
4. Formalin-fixed, paraffin-embedded mouse lung tissue stained for Phospho-MCU (S92) using 14297 at 1/100 dilution in immunohistochemical analysis.
5. Formalin-fixed, paraffin-embedded rat lung tissue stained for Phospho-MCU (S92) using 14297 at 1/100 dilution in immunohistochemical analysis.
6. Immunocytochemistry/Immunofluorescence-Phospho-MCU (S92) antibody, Phospho-MCU (S92) Rabbit mAb staining Phospho-MCU (S92) in the HeLa cell line by ICC/IF. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with Phospho-MCU (S92) Rabbit mAb at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. The negative control is shown in bottom right hand panel-for the negative control, purified Phospho-MCU (S92) Rabbit mAb was used at a dilution of 1/100 followed by Alexa Fluor® 647 goat anti- rabbit antibody at a dilution of 1/500. Nuclei were counterstained with DAPI.

WB: 1:500-2000; IHC: 1:50-200; IF: 1:50-200

Polyclonal

Unconjugated

Phospho

A synthesized phosphopeptide: human MCU Receptor around the phosphorylation site of S92

human