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Anti-Phospho-MAP2K1 (Ser221) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-MAP2K1 (Ser221)。Anti-Phospho-MAP2K1 (Ser221) Polyclonal Antibody 可用于 WB,IHC,IF。
Anti-Phospho-MAP2K1 (Ser221) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-MAP2K1 (Ser221)。Anti-Phospho-MAP2K1 (Ser221) Polyclonal Antibody 可用于 WB,IHC,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,485 | 5日内发货 | |
100 μL | ¥ 2,245 | 5日内发货 |
产品描述 | Anti-Phospho-MAP2K1 (Ser221) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MAP2K1 (Ser221). Anti-Phospho-MAP2K1 (Ser221) Polyclonal Antibody can be used in WB,IHC,IF. |
别名 | p-MAP2K1 (Ser221), p-MAP2K1 (S221), MAP2K1 (p-Ser221), MAP2K1 (p-S221) |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of extracts from Hela cells untreated or treated with EGF using MEK1 (Phospho-Ser221) Antibody TMAC-02533. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1 (Phospho-Ser221) Antibody TMAC-02533 (left) or the same antibody preincubated with blocking peptide (right). 3. Immunofluorescence staining of methanol-fixed Hela cells using MEK1 (Phospho-Ser221) Antibody TMAC-02533. 4. Western blot analysis of extracts from Hela cells, treated with calf intestinal phosphatase (CIP), using MEK1 (Phospho-Ser221) Antibody TMAC-02533. |
应用 | |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
构建方式 | Polyclonal Antibody |
纯化方式 | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
性状 | Liquid |
缓冲液 | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases. |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | Peptide sequence around phosphorylation site of serine 221 (A-N-S(p)-F-V) derived from Human MEK1 |
抗原种属 | human |
Uniprot ID |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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