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顶部生物活性储存和溶解信息计算器

Anti-Phospho-MAP2K1 (Ser218, 222) Antibody (3S158)

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Anti-Phospho-MAP2K1 (Ser218, 222) Antibody (3S158)
产品编号 TMAC-03237
别名 p-MAP2K1 (Ser218, 222), p-MAP2K1 (S218, 222), MAP2K1 (p-Ser218, 222), MAP2K1 (p-S218, 222)

Anti-Phospho-MAP2K1 (S218, S222) Antibody (3S158) 是一种 Rabbit 抗体，靶向 Phospho-MAP2K1 (S218, S222)。Anti-Phospho-MAP2K1 (S218, S222) Antibody (3S158) 可用于 WB,ICC/IF,IHC,IP。

Anti-Phospho-MAP2K1 (Ser218, 222) Antibody (3S158)

Anti-Phospho-MAP2K1 (Ser218, 222) Antibody (3S158)

Rating icon 还可以
Anti-Phospho-MAP2K1 (Ser218, 222) Antibody (3S158)
产品编号 TMAC-03237 别名 p-MAP2K1 (Ser218, 222), p-MAP2K1 (S218, 222), MAP2K1 (p-Ser218, 222), MAP2K1 (p-S218, 222)

Anti-Phospho-MAP2K1 (S218, S222) Antibody (3S158) 是一种 Rabbit 抗体，靶向 Phospho-MAP2K1 (S218, S222)。Anti-Phospho-MAP2K1 (S218, S222) Antibody (3S158) 可用于 WB,ICC/IF,IHC,IP。

规格价格库存数量
50 μL
¥ 1,490
5日内发货
100 μL
¥ 2,495
5日内发货
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产品介绍

生物活性
产品描述
Anti-Phospho-MAP2K1 (Ser218, 222) Antibody (3S158) 是一种 Rabbit 抗体，靶向 Phospho-MAP2K1 (Ser218, 222)。Anti-Phospho-MAP2K1 (Ser218, 222) Antibody (3S158) 可用于 WB,ICC/IF,IHC,IP。
别名p-MAP2K1 (Ser218, 222), p-MAP2K1 (S218, 222), MAP2K1 (p-Ser218, 222), MAP2K1 (p-S218, 222)
Ig Type
IgG
克隆号
3S158
交叉反应
Human
验证活性
1. Western blot analysis of Phospho-MEK1 (S218/S222) on different lysates using anti-Phospho-MEK1 (S218/S222) antibody at 1/1,000 dilution. Positive control: Lane 1: A431, Lane 2: Hela, Lane 3: 293T.
2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-MEK1 (S218/S222) antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Phospho-MEK1 (S218/S222) antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Phospho-MEK1 (S218/S222) antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Phospho-MEK1 (S218/S222) antibody. Counter stained with hematoxylin.
6. ICC staining Phospho-MEK1 (S218/S222) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining Phospho-MEK1 (S218/S222) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. ICC staining Phospho-MEK1 (S218/S222) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
应用
WB
ICC/IF
IHC
IP
推荐剂量
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Activation of extracellular signal-regulated kinase (ERK) or mitogen-activated protein kinase by MEK (mitogen-activated protein kinase or extracellular signal-regulated kinase kinase) is an essential event in the mitogenic growth factor-induced signal transduction pathway. Phosphorylation of MEKs correlates with their ability to phosphorylate and activate ERKs. MEK1 and MEK2 can also be activated by autophosphorylation. Lipopolysaccharide activates many of the MAPK family members of the immediate upstream MAPK activator MEK1, MEK2, and MEK3. In plants, MEK can phosphorylate and activate MAPK, and that Tyr phosphorylation is critical for the catalytic activity of MAPK in plants.
偶联与修饰
偶联
Unconjugated
其他可选修饰符
Phospho
抗原信息
免疫原
A synthesized phosphopeptide: human MEK1 around the phosphorylation site of Ser218 and 222
抗原种属
human
Uniprot ID
Q02750
化学信息
分子量Theoretical: 45 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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关键词

pMAP2K1 (Ser218, 222)pMAP2K1 (S218, 222)MAP2K1 (pSer218, 222)MAP2K1 (pS218, 222)MAP2K1 (phospho-Ser218, 222)MAP2K1 (phospho-S218, 222)

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