Anti-Phospho-MAK (Tyr159) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-MAK (Tyr159). Anti-Phospho-MAK (Tyr159) Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat

1. Sample:
Hela (Human) Cell Lysate at 30 μg
Raw264.7 (Mouse) Cell Lysate at 30 μg
Primary: Anti-phospho-MAK (Tyr159) (TMAB-10926) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 71 kD
Observed band size: 71/66 kD
2. Sample: Raw264.7 (Mouse) Cell Lysate at 30 μg
Primary: Anti-phospho-MAK (Tyr159) (TMAB-10926) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 71 kD
Observed band size: 73 kD
3. Protein: k562 (human) lysate at 40 μg;
Primary: rabbit Anti-phospho-MAK (Tyr159) (TMAB-10926) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1: 5000;
Predicted band size: 71 kD
Observed band size: 71 kD
4. Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-phospho-MAK (Tyr159) Polyclonal Antibody, Unconjugated (TMAB-10926) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
5. Tissue/cell: human prostate tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-phospho-MAK (Tyr159) Polyclonal Antibody, Unconjugated (TMAB-10926) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthesised phosphopeptide: human MAK around the phosphorylation site of Tyr159

Human

Mak

Serine/threonine-protein kinase MAK

Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors (By similarity). Phosphorylates FZR1 in a cell cycle-dependent manner. Plays a role in the transcriptional coactivation of AR. Could play an important function in spermatogenesis. May play a role in chromosomal stability in prostate cancer cells.