Anti-Phospho-JAK3 (Tyr785) Polyclonal Antibody is a Rabbit antibody targeting Phospho-JAK3 (Tyr785). Anti-Phospho-JAK3 (Tyr785) Polyclonal Antibody can be used in WB.

IgG

Mouse (predicted:Human,Rat,Dog,Pig,Cow,Horse)

Sample:
Lane 1: Mouse Lung Lysates
Lane 2: Mouse Blood cell Lysates
Primary: Anti-Phospho-Jak3 (Tyr785) (TMAB-01443) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 125 kDa
Observed band size: 125 kDa

WB: 1:500-2000

Polyclonal

KLH conjugated synthesised phosphopeptide: human Jak3 around the phosphorylation site of Tyr785

Human

JAK3

Tyrosine-protein kinase JAK3

Signal transducers,Tyrosine kinases,STATs,Transcription Factors,Tyrosine Kinases,STATs

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.