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Anti-Phospho-IKK- alpha/IKBKB (Ser176/177) Polyclonal Antibody
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产品编号 TMAC-02126
别名 p-IKK- alpha/IKBKB (Ser176/177), p-IKK- alpha/IKBKB (S176/177), IKK- alpha/IKBKB (p-Ser176/177), IKK- alpha/IKBKB (p-S176/177)
Anti-Phospho-IKK- alpha/IKBKB (Ser176/177) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-IKK- alpha/IKBKB (Ser176/177)。Anti-Phospho-IKK- alpha/IKBKB (Ser176/177) Polyclonal Antibody 可用于 WB,IHC,IF,ELISA。
Anti-Phospho-IKK- alpha/IKBKB (Ser176/177) Polyclonal Antibody
Anti-Phospho-IKK- alpha/IKBKB (Ser176/177) Polyclonal Antibody
还可以产品编号 TMAC-02126 别名 p-IKK- alpha/IKBKB (Ser176/177), p-IKK- alpha/IKBKB (S176/177), IKK- alpha/IKBKB (p-Ser176/177), IKK- alpha/IKBKB (p-S176/177)
Anti-Phospho-IKK- alpha/IKBKB (Ser176/177) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-IKK- alpha/IKBKB (Ser176/177)。Anti-Phospho-IKK- alpha/IKBKB (Ser176/177) Polyclonal Antibody 可用于 WB,IHC,IF,ELISA。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,495 | 5日内发货 | |
| 100 μL | ¥ 2,235 | 5日内发货 |
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| 产品描述 | Anti-Phospho-IKK- alpha/IKBKB (Ser176/177) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IKK- alpha/IKBKB (Ser176/177). Anti-Phospho-IKK- alpha/IKBKB (Ser176/177) Polyclonal Antibody can be used in WB,IHC,IF,ELISA. |
| 别名 | p-IKK- alpha/IKBKB (Ser176/177), p-IKK- alpha/IKBKB (S176/177), IKK- alpha/IKBKB (p-Ser176/177), IKK- alpha/IKBKB (p-S176/177) |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. All Lanes: IKK-alpha/ beta (Phospho-Ser176/177) Antibody at 1/ 500 dilution. Lane1: Jurkat treated with 20ng/ml TNF-α for 30min Cell lysate, Lane2: Huvec treated with 100ng/ml EGF for 30min Cell lysate, Lane3: Hela treated with 100ng/ml EGF for 30min Cell lysate, Lane4: HepG2 treated with 100ng/ml EGF for 30min Cell lysate, Lane5: Mouse Brain Tissue lysate, Lane6:Mouse lung Tissue lysate, Lane7: Rat Liver Tissue lysateLysates/proteins at 40 μg per lane. Secondary: Goat Anti-Rabbit IgG (HRP) at 1/20000 dilution, Predicted band size: 85kDa, Observed band size: 80kDa. |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Polyclonal Antibody |
| 纯化方式 | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 性状 | Liquid |
| 缓冲液 | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | Phospho |
| 免疫原 | Peptide sequence around phosphorylation site of serine 176/177 (Q-G-S(p)-L-C) derived from Human IKK-alpha/beta |
| 抗原种属 | human |
| Uniprot ID |
| 分子量 | Actual: 85 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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