Anti-Phospho-HSP27 (Ser78) Polyclonal Antibody is a Rabbit antibody targeting Phospho-HSP27 (Ser78). Anti-Phospho-HSP27 (Ser78) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.

IgG

Human,Mouse,Rat (predicted:Dog,Cow,Horse,Rabbit)

1. Blank control (blue line): A431 Cells (fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃).
Primary Antibody (green line): Rabbit Anti-Phospho-HSP27 (Ser78) antibody (TMAB-10825);
Dilution: 0.2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC;
Dilution: 1 μg /test.
2. Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-HSP27 (Ser78)) Polyclonal Antibody, Unconjugated (TMAB-10825) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Sample:
Lane 1: MCF-7 (Human) Cell Lysate at 30 μg
Lane 2: U251 (Human) Cell Lysate at 30 μg
Lane 3: U2os (Human) Cell Lysate at 30 μg
Lane 4: A431 (Human) Cell Lysate at 30 μg
Lane 5: Huvec (Human) Cell Lysate at 30 μg
Lane 6: A549 (Human) Cell Lysate at 30 μg
Primary: Anti-Phospho-HSP27 (Ser78) (TMAB-10825) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD
4. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Phospho-HSP27 (Ser78)) polyclonal Antibody, Unconjugated (TMAB-10825) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
5. Sample: Muscle (Mouse) Lysate at 40 μg
Primary: Anti-Phospho-HSP27 (Ser78) (TMAB-10825) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 23 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500; FCM: 0.2μg/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human HSP27 around the phosphorylation site of Ser78

Human

HSPB1

Heat shock protein beta-1

Involved in stress resistance and actin organization.