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顶部生物活性储存和溶解信息计算器

Anti-Phospho-Hsp27/HSPB (Ser78) Antibody (3C571)

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产品编号 TMAC-03227

别名 p-Hsp27/HSPB (Ser78), p-Hsp27/HSPB (S78), Hsp27/HSPB (p-Ser78), Hsp27/HSPB (p-S78)

Anti-Phospho-Hsp27/HSPB (Ser78) Antibody (3C571) 是一种 Rabbit 抗体，靶向 Phospho-Hsp27/HSPB (Ser78)。Anti-Phospho-Hsp27/HSPB (Ser78) Antibody (3C571) 可用于 WB,ICC/IF,IHC,IP。

Anti-Phospho-Hsp27/HSPB (Ser78) Antibody (3C571)

Anti-Phospho-Hsp27/HSPB (Ser78) Antibody (3C571)

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产品编号 TMAC-03227 别名 p-Hsp27/HSPB (Ser78), p-Hsp27/HSPB (S78), Hsp27/HSPB (p-Ser78), Hsp27/HSPB (p-S78)

Anti-Phospho-Hsp27/HSPB (Ser78) Antibody (3C571) 是一种 Rabbit 抗体，靶向 Phospho-Hsp27/HSPB (Ser78)。Anti-Phospho-Hsp27/HSPB (Ser78) Antibody (3C571) 可用于 WB,ICC/IF,IHC,IP。

规格价格库存数量
50 μL
¥ 1,485
5日内发货
100 μL
¥ 2,495
5日内发货
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产品介绍

生物活性
产品描述
Anti-Phospho-Hsp27/HSPB (Ser78) Antibody (3C571) is a Rabbit antibody targeting Phospho-Hsp27/HSPB (Ser78). Anti-Phospho-Hsp27/HSPB (Ser78) Antibody (3C571) can be used in ICC/IF,IHC,IP,WB.
别名p-Hsp27/HSPB (Ser78), p-Hsp27/HSPB (S78), Hsp27/HSPB (p-Ser78), Hsp27/HSPB (p-S78)
Ig Type
IgG
克隆号
3C571
交叉反应
Human
验证活性
1. Western blot analysis of Phospho-Hsp27 (S78) on different lysates using anti-Phospho-Hsp27 (S78) antibody at 1/1,000 dilution. Positive control: Lane 1: Human heart, Lane 2: Human skeletal muscle.
2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Hsp27 (S78) antibody. Counter stained with hematoxylin.
3. ICC staining Phospho-Hsp27 (S78) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining Phospho-Hsp27 (S78) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining Phospho-Hsp27 (S78) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
verifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivity
应用ICC/IFIHCIPWB
推荐剂量
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景HSP 27 is a constitutively expressed cytoplasmic protein that co-localizes to the nucleus upon stress-induced insult. Heat shock, cytokines and hormones are among the factors that stimulate the synthesis of HSP 27. The intracellular concentration of the mammalian heat shock protein, HSP 27, increases several-fold after heat shock and other metabolic stresses, and is closely associated with the acquisition of thermotolerance. MAP kinase-activated protein kinase-2 phosphorylates HSP 27 on Serine residues Ser 15, Ser 78 and Ser 82, which are phosphorylated in vivo in response to growth factors and heat shock. Ser 15, Ser 78 and Ser 82 occur in the sequence motif RXXS, which is recognized by ribosomal protein S6 kinase II.
偶联与修饰
偶联
Unconjugated
其他可选修饰符
Phospho
抗原信息
免疫原
A synthesized phosphopeptide: human Hsp27 around the phosphorylation site of Ser78
抗原种属
human
Uniprot ID
P04792
化学信息
分子量Theoretical: 27 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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关键词

pHsp27/HSPB (Ser78)pHsp27/HSPB (S78)Hsp27/HSPB (pSer78)Hsp27/HSPB (pS78)Hsp27/HSPB (phospho-Ser78)Hsp27/HSPB (phospho-S78)

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