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Anti-Phospho-Histone H3 (Ser10) Antibody (5C369)
还可以产品编号 TMAY-01536
Anti-Phospho-Histone H3 (Ser10) Antibody (5C369) 是一种 Rabbit 抗体，靶向 Phospho-Histone H3 (Ser10)。Anti-Phospho-Histone H3 (Ser10) Antibody (5C369) 可用于 WB。
Anti-Phospho-Histone H3 (Ser10) Antibody (5C369)
Anti-Phospho-Histone H3 (Ser10) Antibody (5C369)
还可以产品编号 TMAY-01536
Anti-Phospho-Histone H3 (Ser10) Antibody (5C369) 是一种 Rabbit 抗体，靶向 Phospho-Histone H3 (Ser10)。Anti-Phospho-Histone H3 (Ser10) Antibody (5C369) 可用于 WB。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Phospho-Histone H3 (Ser10) Antibody (5C369) is a Rabbit antibody targeting Phospho-Histone H3 (Ser10). Anti-Phospho-Histone H3 (Ser10) Antibody (5C369) can be used in WB. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 5C369 |
| 交叉反应 | Human; Species predicted to react based on 100% sequence homology: mouse, rat |
| 验证活性 | 1. Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with Calyculin A (100 nM, 30 min; +), using Phospho-Histone H3 (Ser10)rabbit monoclonal Antibody at 1:10000 dilution. 2. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with calyculin A (100 nM, 30min; +) (line B); treated with calyculin A and λ-phosphatase (line C) using Phospho-Histone H3 (Ser10) rabbit monoclonal Antibody at 1:20000 dilution. (Validation Experiment) 3. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with calyculin A (100 nM, 30min; +) (line B), using Phospho-Histone H3 (Ser10) rabbit monoclonal Antibody at 1:20000 dilution (upper) or anti-Histone H3 antibody (lower). (Validation Experiment) |
| 应用 | WB |
| 推荐剂量 | WB: 1:10000-1:50000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to residues around Ser10 of Human Phospho-Histone H3. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| 研究背景 | Histone H3.1, also known as HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, is a member of the histone H3 family which is a core component of nucleosome. It is expressed during the S phase, then expression strongly decreases as cell division slows down during the process of differentiation. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher-order chromatin structures. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide corresponding to residues around Ser10 of Human Phospho-Histone H3 |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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