Anti-Phospho-GSK3B (Tyr216) Polyclonal Antibody is a Rabbit antibody targeting Phospho-GSK3B (Tyr216). Anti-Phospho-GSK3B (Tyr216) Polyclonal Antibody can be used in WB.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,GuineaPig)

Sample:
Lane 1: Muscle (Mouse) Lysate at 40 μg
Lane 2: Liver (Mouse) Lysate at 40 μg
Lane 3: Cerebrum (Mouse) Lysate at 40 μg
Lane 4: Pancreas (Mouse) Lysate at 40 μg
Lane 5: Thymus (Mouse) Lysate at 40 μg
Lane 6: Muscle (Rat) Lysate at 40 μg
Lane 7: Liver (Rat) Lysate at 40 μg
Lane 8: Cerebrum (Rat) Lysate at 40 μg
Lane 9: Pancreas (Rat) Lysate at 40 μg
Lane 10: Thymus (Rat) Lysate at 40 μg
Lane 11: MCF-7 (Human) Cell Lysate at 30 μg
Lane 12: A431 (Human) Cell Lysate at 30 μg
Lane 13: U2os (Human) Cell Lysate at 30 μg
Primary: Anti-phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) (TMAB-01417) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47/51 kDa
Observed band size: 47 kDa

WB: 1:500-2000

Polyclonal

KLH conjugated Synthesised phosphopeptide: human GSK-3 Beta around the phosphorylation site of Tyr216

Human

GSK3B

Glycogen synthase kinase-3 beta

Integration of energy metabolism,Metabolism of carbohydrates,Diabetes associated,Hypertrophy,Carbohydrate metabolism,Integration of energy,Cancer,Diabetes,Heart disease,Notch Pathway,Other Kinases,Cytoplasmic,Cytoplasmic

Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase. In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates NFATC1/NFATC on conserved serine residues promoting NFATC1/NFATC nuclear export, shutting off NFATC1/NFATC gene regulation, and thereby opposing the action of calcineurin. Phosphorylates MAPT/TAU on 'Thr-548', decreasing significantly MAPT/TAU ability to bind and stabilize microtubules. MAPT/TAU is the principal component of neurofibrillary tangles in Alzheimer disease. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Phosphorylates MACF1, inhibiting its binding to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. Probably regulates NF-kappa-B (NFKB1) at the transcriptional level and is required for the NF-kappa-B-mediated anti-apoptotic response to TNF-alpha (TNF/TNFA). Negatively regulates replication in pancreatic beta-cells, resulting in apoptosis, loss of beta-cells and diabetes. Phosphorylates MUC1 in breast cancer cells, decreasing the interaction of MUC1 with CTNNB1/beta-catenin. Is necessary for the establishment of neuronal polarity and axon outgrowth. Phosphorylates MARK2, leading to inhibit its activity. Phosphorylates SIK1 at 'Thr-182', leading to sustain its activity.