Anti-Phospho-FRA1 (Ser265) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-FRA1 (Ser265). Anti-Phospho-FRA1 (Ser265) Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit)

1. Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Phospho-FRA1 (Ser265) Polyclonal Antibody, Unconjugated (TMAB-10719) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Hela (Human) Cell Lysate at 30 μg
U87Mg (Human) Cell Lysate at 30 μg
Primary: Anti-Phospho-FRA1 (Ser265) (TMAB-10719) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
3. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-FRA1 (Ser265)) Polyclonal Antibody, Unconjugated (TMAB-10719) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human FRA1 around the phosphorylation site of Ser265

Human

FOSL1

Fos-related antigen 1