Anti-Phospho-FOXO1+FOXO3+FOXO4 (Thr24+Thr32) Polyclonal Antibody is a Rabbit antibody targeting Phospho-FOXO1+FOXO3+FOXO4 (Thr24+Thr32). Anti-Phospho-FOXO1+FOXO3+FOXO4 (Thr24+Thr32) Polyclonal Antibody can be used in FCM.

IgG

Human (predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,Rabbit)

1. Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-Phospho-FOXO1+FOXO3+FOXO4 (phospho T24+T32) antibody (TMAB-10713)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 2μg/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human FOXO1 around the phosphorylation site of Thr24

Human

FOXO1

Forkhead box protein O1

Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. Triggers death of postmitotic neurons when phosphorylated by CDK1. Activates transcription of PMAIP1.