Anti-Phospho-FHOD1 (Thr1141) Polyclonal Antibody is a Rabbit antibody targeting Phospho-FHOD1 (Thr1141). Anti-Phospho-FHOD1 (Thr1141) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Rat (predicted:Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit)

1. Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-FHOD1 Polyclonal Antibody, Unconjugated (TMAB-10703) 1: 600, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: Rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-phospho-FHOD1 Polyclonal Antibody, Unconjugated (TMAB-10703) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Sample:
k562 (human) Cell Lysate at 30 μg
Hela (Human) Cell Lysate at 30 μg
Primary: Anti-phospho-FHOD1 (Thr1141) (TMAB-10703) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 126 kD
Observed band size: 126 kD
4. Sample: 293T (Human) Cell Lysate at 30 μg
Primary: Anti-phospho-FHOD1 (Thr1141) (TMAB-10703) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 126 kD
Observed band size: 126 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthesised phosphopeptide: human FHOD1 around the phosphorylation site of Thr1141

Human

FHOD1

FH1/FH2 domain-containing protein 1

Required for the assembly of F-actin structures, such as stress fibers. Depends on the Rho-ROCK cascade for its activity. Contributes to the coordination of microtubules with actin fibers and plays a role in cell elongation.