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Anti-Phospho-ETS1 (Thr38) Polyclonal Antibody 2 是一种 Rabbit 抗体,靶向 Phospho-ETS1 (Thr38)。Anti-Phospho-ETS1 (Thr38) Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-Phospho-ETS1 (Thr38) Polyclonal Antibody 2 是一种 Rabbit 抗体,靶向 Phospho-ETS1 (Thr38)。Anti-Phospho-ETS1 (Thr38) Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,160 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,780 | 5日内发货 |
| 产品描述 | Anti-Phospho-ETS1 (Thr38) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-ETS1 (Thr38). Anti-Phospho-ETS1 (Thr38) Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 反应种属 | Human,Mouse |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-ETS1 (Thr38)) Polyclonal Antibody, Unconjugated (TMAB-10680) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Sample: HepG2 (Human) Cell Lysate at 30 μg Spleen (Mouse) Lysate at 40 μg Lymph node (Mouse) Lysate at 40 μg JurkaT (Human) Cell Lysate at 30 μg Primary: Anti-phospho-ETS1 (Thr38) (TMAB-10680) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kD Observed band size: 50 kD |
| 应用 | WBIHC-PIHC-FrIF |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Nucleus. |
| 构建方式 | Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 浓度 | 1mg/ml |
| 研究背景 | This gene encodes a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. These proteins function either as transcriptional activators or repressors of numerous genes, and are involved in stem cell development, cell senescence and death, and tumorigenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]. |
| 免疫原 | KLH conjugated synthesised phosphopeptide: human ETS1 around the phosphorylation site of Thr38 |
| 抗原种属 | Human |
| 基因名称 | ETS1 |
| 基因ID | |
| 蛋白名称 | Protein C-ets-1 |
| Uniprot ID | |
| 功能 | Transcription factor. |
| 分子量 | Theoretical: 49 kDa. |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |