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Anti-Phospho-ERK1/2 (Thr202, Tyr204) Antibody (4P145)
还可以产品编号 TMAY-01512
Anti-Phospho-ERK1/2 (Thr202, Tyr204) Antibody (4P145) 是一种 Rabbit 抗体，靶向 Phospho-ERK1/2 (Thr202, Tyr204)。Anti-Phospho-ERK1/2 (Thr202, Tyr204) Antibody (4P145) 可用于 WB。
Anti-Phospho-ERK1/2 (Thr202, Tyr204) Antibody (4P145)
Anti-Phospho-ERK1/2 (Thr202, Tyr204) Antibody (4P145)
还可以产品编号 TMAY-01512
Anti-Phospho-ERK1/2 (Thr202, Tyr204) Antibody (4P145) 是一种 Rabbit 抗体，靶向 Phospho-ERK1/2 (Thr202, Tyr204)。Anti-Phospho-ERK1/2 (Thr202, Tyr204) Antibody (4P145) 可用于 WB。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Phospho-ERK1/2 (Thr202, Tyr204) Antibody (4P145) is a Rabbit antibody targeting Phospho-ERK1/2 (Thr202, Tyr204). Anti-Phospho-ERK1/2 (Thr202, Tyr204) Antibody (4P145) can be used in WB. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 4P145 |
| 交叉反应 | Human |
| 验证活性 | 1. Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with Calyculin A (100 nM, 30 min; +), using Phospho-p44/42 MAPK (ERK1/2) (Thr202, Tyr204) rabbit monoclonal Antibody (upper), or Anti-ERK2 rabbit polyclonal antibody at 1:1000 dilution (lower). 2. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with Calyculin A (100 nM, 30 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-p44/42 MAPK (ERK1/2) (Thr202, Tyr204) rabbit monoclonal Antibody at 1:1000 dilution. (Validation Experiment) 3. Western blot analysis of 15 μg extracts from serum-starved NIH-3T3, untreated (-); treated with PDGFA (5 μg/mL, 5 min; +), using Phospho-ERK1/2 (Thr202, Tyr204) Antibody, Rabbit MAb at 1:1000 dilution. (Validation Experiment) 4. Western blot analysis of extracts from serum-starved Hela, treated with Calyculin A (100 nM, 30 min), using Phospho-p44/42 MAPK (ERK1/2) (Thr202, Tyr204) rabbit monoclonal Antibody and other brands’ antibodies (company C) at dilution of 1:1000, 1:10000 and 1:200000. (Validation Experiment) |
| 应用 | WB |
| 推荐剂量 | WB: 1:1000-1:20000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around Thr202, Tyr204 of Human Phospho-ERK1/2. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| 研究背景 | MAPK3 (Mitogen-Activated Protein Kinase 3, also known as ERK1) is a Protein Coding gene. The protein encoded by the ERK1 gene is a member of the MAP kinase family. 3 alternatively spliced human isoforms have been reported. The protein is a serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs that play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. ERK1 is widely expressed in the small intestine, colon, and other tissues. Diseases associated with MAPK3 include Neutrophil Migration and Pertussis.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic phosphopeptide corresponding to residues around Thr202, Tyr204 of Human Phospho-ERK1/2. |
| 抗原种属 | Human |
| 研究领域 | Cancer Drug Targets |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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