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Anti-Phospho-EphB1 (Tyr928) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-EphB1 (Tyr928)。Anti-Phospho-EphB1 (Tyr928) Polyclonal Antibody 可用于 WB,IHC,IF。
Anti-Phospho-EphB1 (Tyr928) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-EphB1 (Tyr928)。Anti-Phospho-EphB1 (Tyr928) Polyclonal Antibody 可用于 WB,IHC,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,480 | 5日内发货 | |
100 μL | ¥ 2,490 | 5日内发货 |
产品描述 | Anti-Phospho-EphB1 (Tyr928) Polyclonal Antibody is a Rabbit antibody targeting Phospho-EphB1 (Tyr928). Anti-Phospho-EphB1 (Tyr928) Polyclonal Antibody can be used in WB,IHC,IF. |
别名 | p-EphB1 (Y928), p-EphB1 (Tyr928), EphB1 (p-Y928), EphB1 (p-Tyr928) |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Sample: Ovary (Mouse) Lysate at 40 ug, Primary: at 1/300 dilution, Secondary: at 1/20000 dilution, Predicted band size: 106 kD, Observed band size: 121 kD. 2. Sample: Lane 1: Mouse Testis tissue lysate, sLane 2: Mouse NIH/3T3 cell lysates, Lane 3: Human U251 cell lysates, Lane 4: Human U87MG cell lysates, Lane 5: Human MCF-7 cell lysates, Lane 6: Human U-2 OS cell lysates, Primary: at 1/1000 dilution, Secondary: at 1/20000 dilution, Predicted band size: 106 kDa, Observed band size: 130 kDa. 3. Tissue/cell: Rat brain; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Primary:1:200, overnight at 4°C. |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC: 1:100-500; IF: 1:100-500 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
构建方式 | Polyclonal Antibody |
纯化方式 | affinity purified by Protein A |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | KLH conjugated Synthesised phosphopeptide: derived from human EphB1 around the phosphorylation site of Tyr928: VQ(p-Y)RD |
抗原种属 | human |
Uniprot ID |
分子量 | Theoretical: 106 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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