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Anti-Phospho-Cyclin H (Thr315) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-Cyclin H (Thr315)。Anti-Phospho-Cyclin H (Thr315) Polyclonal Antibody 可用于 WB。
Anti-Phospho-Cyclin H (Thr315) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-Cyclin H (Thr315)。Anti-Phospho-Cyclin H (Thr315) Polyclonal Antibody 可用于 WB。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,485 | 5日内发货 | |
100 μL | ¥ 2,240 | 5日内发货 |
产品描述 | Anti-Phospho-Cyclin H (Thr315) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Cyclin H (Thr315). Anti-Phospho-Cyclin H (Thr315) Polyclonal Antibody can be used in WB. |
别名 | p-Cyclin H (Thr315), p-Cyclin H (T315), Cyclin H (p-Thr315), Cyclin H (p-T315) |
Ig Type | IgG |
交叉反应 | Human,Mouse |
验证活性 | 1. Western blot analysis of extracts from JK cells (Lane 2), using Cyclin H (Phospho-Thr315) Antibody TMAC-01050. The lane on the left is treated with antigen-specific peptide. |
应用 | |
推荐剂量 | WB: 1:500-1000 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
构建方式 | Polyclonal Antibody |
纯化方式 | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
性状 | Liquid |
缓冲液 | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle. |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | Peptide sequence around phosphorylation site of threonine 315 (E-W-T(p)-D-D) derived from Human Cyclin H |
抗原种属 | human |
Uniprot ID |
分子量 | Actual: 36 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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