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Anti-Phospho-CK1 alpha (Tyr294) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-CK1 alpha (Tyr294)。Anti-Phospho-CK1 alpha (Tyr294) Polyclonal Antibody 可用于 WB,IHC。
Anti-Phospho-CK1 alpha (Tyr294) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-CK1 alpha (Tyr294)。Anti-Phospho-CK1 alpha (Tyr294) Polyclonal Antibody 可用于 WB,IHC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,480 | 5日内发货 | |
100 μL | ¥ 2,230 | 5日内发货 |
产品描述 | Anti-Phospho-CK1 alpha (Tyr294) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CK1 alpha (Tyr294). Anti-Phospho-CK1 alpha (Tyr294) Polyclonal Antibody can be used in WB,IHC. |
别名 | p-CK1 alpha (Y294), p-CK1 alpha (Tyr294), CK1 alpha (p-Y294), CK1 alpha (p-Tyr294) |
Ig Type | IgG |
交叉反应 | Human |
验证活性 | 1. Western blot analysis of extracts from HT-29 cells treated with heat shock using CK-1α (Phospho-Tyr294) Antibody TMAC-00870.The lane on the right is treated with the antigen-specific peptide. 2. Immunohistochemical analysis of paraffin-embedded human brain tissue using CK-1α (Phospho-Tyr294) antibody TMAC-00870 (left)or the same antibody preincubated with blocking peptide (right). |
应用 | |
推荐剂量 | WB: 1:500-1000; IHC: 1:50-100 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
构建方式 | Polyclonal Antibody |
纯化方式 | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
性状 | Liquid |
缓冲液 | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis. |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | Peptide sequence around phosphorylation site of tyrosine 294 (Y-D-Y(p)-T-F) derived from Human CK-1α |
抗原种属 | human |
Uniprot ID |
分子量 | Actual: 37 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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