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顶部生物活性储存和溶解信息计算器

Anti-Phospho-CHEK1 (Ser280) Polyclonal Antibody

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Anti-Phospho-CHEK1 (Ser280) Polyclonal Antibody
产品编号 TMAC-00840
别名 p-CHEK1 (Ser280), p-CHEK1 (S280), CHEK1 (p-Ser280), CHEK1 (p-S280)

Anti-Phospho-CHEK1 (Ser280) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-CHEK1 (Ser280)。Anti-Phospho-CHEK1 (Ser280) Polyclonal Antibody 可用于 WB。

Anti-Phospho-CHEK1 (Ser280) Polyclonal Antibody

Anti-Phospho-CHEK1 (Ser280) Polyclonal Antibody

Rating icon 还可以
Anti-Phospho-CHEK1 (Ser280) Polyclonal Antibody
产品编号 TMAC-00840 别名 p-CHEK1 (Ser280), p-CHEK1 (S280), CHEK1 (p-Ser280), CHEK1 (p-S280)

Anti-Phospho-CHEK1 (Ser280) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-CHEK1 (Ser280)。Anti-Phospho-CHEK1 (Ser280) Polyclonal Antibody 可用于 WB。

规格价格库存数量
50 μL
¥ 1,485
5日内发货
100 μL
¥ 2,230
5日内发货
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产品介绍

生物活性
产品描述
Anti-Phospho-CHEK1 (Ser280) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CHEK1 (Ser280). Anti-Phospho-CHEK1 (Ser280) Polyclonal Antibody can be used in WB.
别名p-CHEK1 (Ser280), p-CHEK1 (S280), CHEK1 (p-Ser280), CHEK1 (p-S280)
Ig Type
IgG
交叉反应
Human
验证活性
1. Western blot analysis of extracts from HepG2 cells untreated or treated with UV using Chk1 (Phospho-Ser280) Antibody TMAC-00840.
2. Western blot analysis of extracts from A2780 cells, treated with calf intestinal phosphatase (CIP), using Chk1 (Phospho-Ser280) Antibody TMAC-00840.
应用
WB
抗体种类
Polyclonal
宿主来源Rabbit
构建方式Polyclonal Antibody
纯化方式Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
性状Liquid
缓冲液Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.
偶联与修饰
偶联
Unconjugated
其他可选修饰符
Phospho
抗原信息
免疫原
Peptide sequence around phosphorylation site of serine 280 (V-T-S(p)-G-G) derived from Human Chk1
抗原种属
human
Uniprot ID
O14757
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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关键词

pCHEK1 (Ser280)pCHEK1 (S280)CHEK1 (pSer280)CHEK1 (pS280)CHEK1 (phospho-Ser280)CHEK1 (phospho-S280)

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4个月前
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