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Anti-Phospho-CCNE1 (T77) Antibody (8N839) 是一种 Rabbit 抗体,靶向 Phospho-CCNE1 (T77)。Anti-Phospho-CCNE1 (T77) Antibody (8N839) 可用于 WB,ICC/IF,IHC。
Anti-Phospho-CCNE1 (T77) Antibody (8N839) 是一种 Rabbit 抗体,靶向 Phospho-CCNE1 (T77)。Anti-Phospho-CCNE1 (T77) Antibody (8N839) 可用于 WB,ICC/IF,IHC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,480 | 5日内发货 | |
100 μL | ¥ 2,495 | 5日内发货 |
产品描述 | Anti-Phospho-CCNE1 (Thr77) Antibody (8N839) 是一种 Rabbit 抗体,靶向 Phospho-CCNE1 (Thr77)。Anti-Phospho-CCNE1 (Thr77) Antibody (8N839) 可用于 WB,ICC/IF,IHC。 |
别名 | p-CCNE1 (Thr77), p-CCNE1 (T77), CCNE1 (p-Thr77), CCNE1 (p-T77) |
Ig Type | IgG |
交叉反应 | Human |
验证活性 | 1. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-Cyclin E1 (T77) antibody. Counter stained with hematoxylin. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-Cyclin E1 (T77) antibody. Counter stained with hematoxylin. 3. ICC staining Phospho-Cyclin E1 (T77) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining Phospho-Cyclin E1 (T77) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Phospho-Cyclin E1 (T77) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
应用 | |
推荐剂量 | WB: 1:1000; IHC: 1:50-200; ICC/IF: 1:50-200 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. The cyclin E/E2F complex is detected primarily during the G1 phase of the cell cycle and decreases as cells enter S phase. E2F is known to be a critical transcription factor for expression of several S phase specific proteins. |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | A synthesized phosphopeptide: human Cyclin E1 around the phosphorylation site of Thr77 |
抗原种属 | human |
Uniprot ID |
分子量 | Theoretical: 48 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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