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别名 p-CCNE1 (Thr77), p-CCNE1 (T77), CCNE1 (p-Thr77), CCNE1 (p-T77)
Anti-Phospho-CCNE1 (Thr77) Antibody (8N839) 是一种 Rabbit 抗体,靶向 Phospho-CCNE1 (Thr77)。Anti-Phospho-CCNE1 (Thr77) Antibody (8N839) 可用于 WB,ICC/IF,IHC。
Anti-Phospho-CCNE1 (Thr77) Antibody (8N839) 是一种 Rabbit 抗体,靶向 Phospho-CCNE1 (Thr77)。Anti-Phospho-CCNE1 (Thr77) Antibody (8N839) 可用于 WB,ICC/IF,IHC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,480 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
| 产品描述 | Anti-Phospho-CCNE1 (Thr77) Antibody (8N839) is a Rabbit antibody targeting Phospho-CCNE1 (Thr77). Anti-Phospho-CCNE1 (Thr77) Antibody (8N839) can be used in ICC/IF,IHC,WB. |
| 别名 | p-CCNE1 (Thr77), p-CCNE1 (T77), CCNE1 (p-Thr77), CCNE1 (p-T77) |
| Ig Type | IgG |
| 克隆号 | 8N839 |
| 反应种属 | Human |
| 验证活性 | 1. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-Cyclin E1 (T77) antibody. Counter stained with hematoxylin. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-Cyclin E1 (T77) antibody. Counter stained with hematoxylin. 3. ICC staining Phospho-Cyclin E1 (T77) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining Phospho-Cyclin E1 (T77) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Phospho-Cyclin E1 (T77) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. ![]() ![]() ![]() ![]() ![]() |
| 应用 | ICC/IFIHCWB |
| 推荐剂量 | WB: 1:1000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. The cyclin E/E2F complex is detected primarily during the G1 phase of the cell cycle and decreases as cells enter S phase. E2F is known to be a critical transcription factor for expression of several S phase specific proteins. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | Phospho |
| 免疫原 | A synthesized phosphopeptide: human Cyclin E1 around the phosphorylation site of Thr77 |
| 抗原种属 | human |
| Uniprot ID |
| 分子量 | Theoretical: 48 kDa. |
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
| 存储 | store at low temperature | -20°C for 1 year |