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Anti-Phospho-CASP9 (Thr125) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-CASP9 (Thr125)。Anti-Phospho-CASP9 (Thr125) Polyclonal Antibody 可用于 WB,IHC。
Anti-Phospho-CASP9 (Thr125) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-CASP9 (Thr125)。Anti-Phospho-CASP9 (Thr125) Polyclonal Antibody 可用于 WB,IHC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,490 | 5日内发货 | |
100 μL | ¥ 2,235 | 5日内发货 |
产品描述 | Anti-Phospho-CASP9 (Thr125) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CASP9 (Thr125). Anti-Phospho-CASP9 (Thr125) Polyclonal Antibody can be used in WB,IHC. |
别名 | p-CASP9 (Thr125), p-CASP9 (T125), CASP9 (p-Thr125), CASP9 (p-T125) |
Ig Type | IgG |
交叉反应 | Human |
验证活性 | 1. Western blot analysis of extracts from HeLa cells treated with TNF using Caspase 9 (Phospho-Thr125) Antibody TMAC-00573. The lane on the right is treated with the antigen-specific peptide. 2. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Caspase 9 (Phospho-Thr125) antibody TMAC-00573 (left) or the same antibody preincubated with blocking peptide (right). |
应用 | |
推荐剂量 | WB: 1:500-1000; IHC: 1:50-100 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
构建方式 | Polyclonal Antibody |
纯化方式 | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
性状 | Liquid |
缓冲液 | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | Peptide sequence around phosphorylation site of threonine 125 (P-E-T(p)-P-R) derived from Human Caspase 9 |
抗原种属 | human |
Uniprot ID |
分子量 | Actual: 47 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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