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Anti-Phospho-Bad (Ser99) Antibody (7Q57)
还可以产品编号 TMAY-01663
Anti-Phospho-Bad (Ser99) Antibody (7Q57) 是一种 Rabbit 抗体，靶向 Phospho-Bad (Ser99)。Anti-Phospho-Bad (Ser99) Antibody (7Q57) 可用于 WB。
Anti-Phospho-Bad (Ser99) Antibody (7Q57)
Anti-Phospho-Bad (Ser99) Antibody (7Q57)
还可以产品编号 TMAY-01663
Anti-Phospho-Bad (Ser99) Antibody (7Q57) 是一种 Rabbit 抗体，靶向 Phospho-Bad (Ser99)。Anti-Phospho-Bad (Ser99) Antibody (7Q57) 可用于 WB。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Phospho-Bad (Ser99) Antibody (7Q57) is a Rabbit antibody targeting Phospho-Bad (Ser99). Anti-Phospho-Bad (Ser99) Antibody (7Q57) can be used in WB. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 7Q57 |
| 交叉反应 | Human |
| 验证活性 | 1. Western blot analysis of extracts from serum-starved HEK293 overexpressing BAD, untreated(line A) or treated with forskolin (100nM, 30min; +)(line B), using Phospho-Bad (Ser99) rabbit monoclonal Antibody at 1:1000 dilution. 2. Western blot analysis of extracts from serum-starved HEK293 overexpressing BAD, untreated (line A); treated with forskolin (100nM, 30min; +)(line B); treated with forskolin and λ-phosphatase (line C) using Phospho-Bad (Ser99) rabbit monoclonal Antibody at 1:1000 dilution. (Validation Experiment) |
| 应用 | WB |
| 推荐剂量 | WB: 1:1000-1:20000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the residues around Ser99 of Human Phospho-Bad. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| 研究背景 | Bcl-2 agonist of cell death (BAD) is a BH3-only member of the Bcl-2 family which possesses important regulatory function in apoptosis. BAD has also been shown to possess many non-apoptotic functions closely linked to cancer including regulation of glycolysis, autophagy, cell cycle progression and immune system development. Oral squamous cell carcinoma (OSCC) is a type of cancer with high morbidity and mortality rates worldwide; it also demonstrates chemotherapeutic resistance. Triterpenoid ursolic acid has been shown to exhibit various biological activities and anticancer effects in several preclinical studies. The apoptosis‑associated signaling showed that ursolic acid decreased the phosphorylation of AKT (Ser473) and B‑cell lymphoma 2 (Bcl‑2)‑associated agonist of cell death (BAD; Ser136), and the protein levels of Bcl‑2 and Bcl‑extra large (Bcl‑xL), and increased the expression of BAD and Bcl‑2‑associated X (Bax) protein in CAR cells. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide corresponding to the residues around Ser99 of Human Phospho-Bad |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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