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顶部产品介绍偶联与修饰抗原信息化学信息储存和溶解信息计算器

Anti-Phospho-AMPKα (Ser496) Antibody (3X970)

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货号 TMAC-03190

别名 Phospho-AMPKα (S496), p-AMPKα (Ser496), p-AMPKα (S496), AMPKα (p-Ser496), AMPKα (p-S496), AMPKalpha, AMPKa1, AMPK1, AMPK alpha 1, AMPK

Anti-Phospho-AMPKα (Ser496) Antibody (3X970) 是一种 Rabbit 抗体，靶向 Phospho-AMPKα (Ser496)。Anti-Phospho-AMPKα (Ser496) Antibody (3X970) 可用于 WB,ICC/IF,IP。

Anti-Phospho-AMPKα (Ser496) Antibody (3X970)

Anti-Phospho-AMPKα (Ser496) Antibody (3X970)

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货号 TMAC-03190 别名 Phospho-AMPKα (S496), p-AMPKα (Ser496), p-AMPKα (S496), AMPKα (p-Ser496), AMPKα (p-S496), AMPKalpha, AMPKa1, AMPK1, AMPK alpha 1, AMPK

Anti-Phospho-AMPKα (Ser496) Antibody (3X970) 是一种 Rabbit 抗体，靶向 Phospho-AMPKα (Ser496)。Anti-Phospho-AMPKα (Ser496) Antibody (3X970) 可用于 WB,ICC/IF,IP。

规格价格库存数量
50 μL
¥ 1,490
5日内发货
100 μL
¥ 2,495
5日内发货
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产品介绍

生物活性
产品描述
Anti-Phospho-AMPKα (Ser496) Antibody (3X970) is a Rabbit antibody targeting Phospho-AMPKα (Ser496). Anti-Phospho-AMPKα (Ser496) Antibody (3X970) can be used in ICC/IF,IP,WB.
别名Phospho-AMPKα (S496), p-AMPKα (Ser496), p-AMPKα (S496), AMPKα (p-Ser496), AMPKα (p-S496), AMPKalpha, AMPKa1, AMPK1, AMPK alpha 1, AMPK
Ig Type
IgG
克隆号
3X970
反应种属
Human
验证活性
1. Western blot analysis of Phospho-AMPK alpha 1(S496) on 293T cells lysates using anti-Phospho-AMPK alpha 1 (S496) antibody at 1/1,000 dilution.
2. ICC staining Phospho-AMPK alpha 1 (S496) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
3. ICC staining Phospho-AMPK alpha 1 (S496) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining Phospho-AMPK alpha 1 (S496) in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
verifiedActivityverifiedActivityverifiedActivityverifiedActivity
应用ICC/IFIPWB
推荐剂量
WB: 1:1000-5000; ICC/IF: 1:50-200
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景AMPK (5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming bio-synthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. AMPKα1 (5'-AMP-activated protein kinase catalytic subunit alpha-1), also known as PRKAA1, is a 559 amino acid protein that belongs to the CAMK Ser/Thr protein kinase family and protein kinase superfamily. Highly phosphorylated, AMPKα1 exists as two alternatively spliced isoforms and is encoded by a gene that maps to human chromosome 5p13.1.
偶联与修饰
偶联
Unconjugated
其他可选修饰符
Phospho
抗原信息
免疫原
A synthesized phosphopeptide: human AMPK alpha 1 around the phosphorylation site of Ser496
抗原种属
human
Uniprot ID
Q13131
化学信息
分子量Theoretical: 64 kDa.
储存&溶解度
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.
存储store at low temperature | -20°C for 1 year

计算器

  • 稀释 计算器

关键词

pAMPKα (Ser496)pAMPKα (S496)AMPKα (pSer496)AMPKα (pS496)AMPKα (phospho-Ser496)AMPKα (phospho-S496)
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