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Anti-Phospho-ADD1 (Ser726) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-ADD1 (Ser726)。Anti-Phospho-ADD1 (Ser726) Polyclonal Antibody 可用于 WB,IHC,IF。
Anti-Phospho-ADD1 (Ser726) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-ADD1 (Ser726)。Anti-Phospho-ADD1 (Ser726) Polyclonal Antibody 可用于 WB,IHC,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,495 | 5日内发货 | |
100 μL | ¥ 2,235 | 5日内发货 |
产品描述 | Anti-Phospho-ADD1 (Ser726) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ADD1 (Ser726). Anti-Phospho-ADD1 (Ser726) Polyclonal Antibody can be used in WB,IHC,IF. |
别名 | p-ADD1 (Ser726), p-ADD1 (S726), ADD1 (p-Ser726), ADD1 (p-S726) |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of extracts from Hela cells using ADD1(Phospho-Ser726) Antibody TMAC-00089 and the same antibody preincubated with blocking peptide. 2. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ADD1(Phospho-Ser726) Antibody TMAC-00089(left) or the same antibody preincubated with blocking peptide(right). 3. Immunofluorescence staining of methanol-fixed Hela cells using ADD1(Phospho-Ser726) Antibody TMAC-00089. |
应用 | |
推荐剂量 | WB: 1:500-1000; IHC: 1:50-100; IF: 1:100-200 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
构建方式 | Polyclonal Antibody |
纯化方式 | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
性状 | Liquid |
缓冲液 | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | Adducins are a family of cytoskeleton proteins encoded by three genes (a, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. a- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. a- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | Peptide sequence around phosphorylation site of serine 726 (T-P-S(p)-F-L) derived from Human ADD1 |
抗原种属 | human |
Uniprot ID |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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