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顶部生物活性储存和溶解信息计算器

Anti-Phospho-ACK1 (Tyr284) Polyclonal Antibody

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产品编号 TMAC-00070

别名 p-ACK1 (Y284), p-ACK1 (Tyr284), ACK1 (p-Y284), ACK1 (p-Tyr284)

Anti-Phospho-ACK1 (Tyr284) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-ACK1 (Tyr284)。Anti-Phospho-ACK1 (Tyr284) Polyclonal Antibody 可用于 IF,IHC,WB。

Anti-Phospho-ACK1 (Tyr284) Polyclonal Antibody

Anti-Phospho-ACK1 (Tyr284) Polyclonal Antibody

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Rating icon 还可以
产品编号 TMAC-00070 别名 p-ACK1 (Y284), p-ACK1 (Tyr284), ACK1 (p-Y284), ACK1 (p-Tyr284)

Anti-Phospho-ACK1 (Tyr284) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-ACK1 (Tyr284)。Anti-Phospho-ACK1 (Tyr284) Polyclonal Antibody 可用于 IF,IHC,WB。

规格价格库存数量
50 μL
¥ 1,495
5日内发货
100 μL
¥ 2,245
5日内发货
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产品介绍

生物活性
产品描述
Anti-Phospho-ACK1 (Tyr284) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ACK1 (Tyr284). Anti-Phospho-ACK1 (Tyr284) Polyclonal Antibody can be used in IF,IHC,WB.
别名p-ACK1 (Y284), p-ACK1 (Tyr284), ACK1 (p-Y284), ACK1 (p-Tyr284)
Ig Type
IgG
交叉反应
Human,Mouse
验证活性
1. Western blot analysis of extracts from HepG2 cells treated with EGF using ACK1 (Phospho-Tyr284) Antibody TMAC-00070.The lane on the right is treated with the antigen-specific peptide.
2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ACK1 (Phospho-Tyr284) antibody TMAC-00070 (left)or the same antibody preincubated with blocking peptide (right).
3. Immunofluorescence staining of methanol-fixed A549 cells using ACK1 (Phospho-Tyr284) Antibody TMAC-00070.
verifiedActivityverifiedActivityverifiedActivity
应用IFIHCWB
推荐剂量
WB: 1:500-1000; IHC: 1:50-100; IF: 1:100-200
抗体种类
Polyclonal
宿主来源Rabbit
构建方式Polyclonal Antibody
纯化方式Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
性状Liquid
缓冲液Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes.
偶联与修饰
偶联
Unconjugated
其他可选修饰符
Phospho
抗原信息
免疫原
Peptide sequence around phosphorylation site of tyrosine 284 (D-H-Y(p)-V-M) derived from Human ACK1
抗原种属
human
Uniprot ID
Q07912
化学信息
分子量Actual: 120 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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关键词

pACK1 (Y284)pACK1 (Tyr284)ACK1 (pY284)ACK1 (pTyr284)ACK1 (phospho-Y284)ACK1 (phospho-Tyr284)

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