Anti-PHGDH Antibody (2P360) is a Mouse antibody targeting PHGDH. Anti-PHGDH Antibody (2P360) can be used in WB,IHC-P,IHC-Fr,IF.

IgG1, Kappa

2P360

Human,Rat,Mouse

1. Paraformaldehyde-fixed, paraffin embedded Rat Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PHGDH Monoclonal Antibody, Unconjugated (TMAB-10268) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PHGDH Monoclonal Antibody, Unconjugated (TMAB-10268) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PHGDH Monoclonal Antibody, Unconjugated (TMAB-10268) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PHGDH Monoclonal Antibody, Unconjugated (TMAB-10268) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PHGDH Monoclonal Antibody, Unconjugated (TMAB-10268) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PHGDH Monoclonal Antibody, Unconjugated (TMAB-10268) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human fallopian tube; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PHGDH Monoclonal Antibody, Unconjugated (TMAB-10268) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PHGDH Monoclonal Antibody, Unconjugated (TMAB-10268) at 1: 200 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
9. 25 μg total protein per lane of various lysates (see on figure) probed with PHGDH monoclonal antibody, unconjugated (TMAB-10268) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

PHGDH

D-3-phosphoglycerate dehydrogenase

Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.