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顶部产品介绍抗原信息化学信息储存和溶解信息计算器

Anti-PHAP1 Polyclonal Antibody

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货号 TMAB-10254

Anti-PHAP1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 PHAP1。Anti-PHAP1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-PHAP1 Polyclonal Antibody

Anti-PHAP1 Polyclonal Antibody

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Rating icon 还可以
货号 TMAB-10254

Anti-PHAP1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 PHAP1。Anti-PHAP1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,175
5日内发货
100 μL
¥ 1,975
5日内发货
200 μL
¥ 2,785
5日内发货
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产品介绍

生物活性
产品描述
Anti-PHAP1 Polyclonal Antibody is a Rabbit antibody targeting PHAP1. Anti-PHAP1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
反应种属
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit)
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (PHAP1) Polyclonal Antibody, Unconjugated (TMAB-10254) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (PHAP1) Polyclonal Antibody, Unconjugated (TMAB-10254) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (PHAP1) Polyclonal Antibody, Unconjugated (TMAB-10254) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
4. Sample:
Cerebrum (Mouse) Lysate at 40 μg
Cerebrum (Rat) Lysate at 40 μg
Cerebellum (Mouse) Lysate at 40 μg
Cerebellum (Rat) Lysate at 40 μg
Primary: Anti-PHAP1 (TMAB-10254) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD
5. Sample: 293T (Human) Cell Lysate at 30 μg
Primary: Anti-PHAP1 (TMAB-10254) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 25 kD
应用WBIHC-PIHC-FrIF
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Nucleus. Cytoplasm. Endoplasmic reticulum. Note=Translocates to the cytoplasm during the process of neuritogenesis (By similarity). Shuttles between nucleus and cytoplasm.
组织特异性Expressed in all tissues tested. Highly expressed in kidney and skeletal muscle, moderate levels of expression in brain, placenta and pancreas, and weakly expressed in lung. Found in all regions of the brain examined (amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus), with highest levels in amygdala.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression.
抗原信息
免疫原
KLH conjugated synthetic peptide: human PHAP1
抗原种属
Human
基因名称
ANP32A
基因ID
8125
蛋白名称
Acidic leucine-rich nuclear phosphoprotein 32 family member A
Uniprot ID
P39687
化学信息
分子量Theoretical: 27 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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