Anti-PFKM Polyclonal Antibody is a Rabbit antibody targeting PFKM. Anti-PFKM Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Rabbit,Cow,Pig,Chicken)

1. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PFKM PFK1) Polyclonal Antibody, Unconjugated (TMAB-01374) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PFKM PFK1) Polyclonal Antibody, Unconjugated (TMAB-01374) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human HUVEC cell lysates
Lane 3: Human U251 cell lysates
Primary: Anti-PFKM/PFK1 (TMAB-01374) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 86 kDa
Observed band size: 85 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human 6 Phosphofructokinase Muscle Type

Human

Metabolism of carbohydrates,Carbohydrate metabolism,Energy Metabolism,Energy Metabolism