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Anti-PFKFB3 Polyclonal Antibody
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产品编号 TMAB-01373
Anti-PFKFB3 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 PFKFB3。Anti-PFKFB3 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-PFKFB3 Polyclonal Antibody
Anti-PFKFB3 Polyclonal Antibody
还可以产品编号 TMAB-01373
Anti-PFKFB3 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 PFKFB3。Anti-PFKFB3 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,970 | 5日内发货 | |
| 200 μL | ¥ 2,795 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-PFKFB3 Polyclonal Antibody is a Rabbit antibody targeting PFKFB3. Anti-PFKFB3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat (predicted:Pig,Cow,Horse,Rabbit,GuineaPig) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PFKFB3/PFK2) Polyclonal Antibody, Unconjugated (TMAB-01373) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PFKFB3/PFK2) Polyclonal Antibody, Unconjugated (TMAB-01373) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Sample: Lane 1: Human HepG2 cell lysates Lane 2: Human HeLa cell lysates Lane 3: Human U251 cell lysates Lane 4: Human A431 cell lysates Lane 5: Human Jurkat cell lysates Primary: Anti-PFKFB3/PFK2 (TMAB-01373) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kDa Observed band size: 60 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016] |
| 免疫原 | KLH conjugated synthetic peptide: human PFKFB3 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Metabolism of carbohydrates,Hypoxia-Regulated,Carbohydrate metabolism,Energy Metabolism,Hypoxia,Metabolism,Energy Metabolism |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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