Anti-PFKFB3 Polyclonal Antibody is a Rabbit antibody targeting PFKFB3. Anti-PFKFB3 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse,Rat (predicted:Pig,Cow,Horse,Rabbit,GuineaPig)

1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PFKFB3/PFK2) Polyclonal Antibody, Unconjugated (TMAB-01373) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PFKFB3/PFK2) Polyclonal Antibody, Unconjugated (TMAB-01373) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human HeLa cell lysates
Lane 3: Human U251 cell lysates
Lane 4: Human A431 cell lysates
Lane 5: Human Jurkat cell lysates
Primary: Anti-PFKFB3/PFK2 (TMAB-01373) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human PFKFB3

Human

PFKFB3

6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3

Metabolism of carbohydrates,Hypoxia-Regulated,Carbohydrate metabolism,Energy Metabolism,Hypoxia,Metabolism,Energy Metabolism

Synthesis and degradation of fructose 2,6-bisphosphate.