Anti-PFKFB2/PFK2 Polyclonal Antibody is a Rabbit antibody targeting PFKFB2/PFK2. Anti-PFKFB2/PFK2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog)

1. Sample:
PC-3 Cell Lysate at 40 μg
HepG2 Cell Lysate at 40 μg
Primary: Anti-PFKFB2/PFK2 (TMAB-10222) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 62 kD
2. Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-PFKFB2 Polyclonal Antibody, Unconjugated (TMAB-10222) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Tissue/cell: Rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-PFKFB2 Polyclonal Antibody, Unconjugated (TMAB-10222) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
4. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PFKFB2) Polyclonal Antibody, Unconjugated (TMAB-10222) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human PFKFB2

Human

PFKFB2

6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2

Synthesis and degradation of fructose 2,6-bisphosphate.