购物车
  • 全部删除
  • TargetMol
    您的购物车当前为空
顶部生物活性储存和溶解信息计算器

Anti-Peroxiredoxin 5 Antibody (6B923)

Rating icon 还可以
Anti-Peroxiredoxin 5 Antibody (6B923)
产品编号 TMAY-01803
别名 PrxV, Prdx6, Pmp20, peroxiredoxin 5, AOPP, AOEB166

Anti-Peroxiredoxin 5 Antibody (6B923) 是一种 Rabbit 抗体，靶向 Peroxiredoxin 5。Anti-Peroxiredoxin 5 Antibody (6B923) 可用于 WB,ICC/IF,IP。

Anti-Peroxiredoxin 5 Antibody (6B923)
331

Anti-Peroxiredoxin 5 Antibody (6B923)

Rating icon 还可以
Anti-Peroxiredoxin 5 Antibody (6B923)
产品编号 TMAY-01803 别名 PrxV, Prdx6, Pmp20, peroxiredoxin 5, AOPP, AOEB166

Anti-Peroxiredoxin 5 Antibody (6B923) 是一种 Rabbit 抗体，靶向 Peroxiredoxin 5。Anti-Peroxiredoxin 5 Antibody (6B923) 可用于 WB,ICC/IF,IP。

规格价格库存数量
100 μL
¥ 2,284
5日内发货
大包装 & 定制
加入购物车
TargetMol 的所有产品仅用作科学研究或药证申报，不能被用于人体，我们不向个人提供产品和服务。请您遵守承诺用途，不得违反法律法规规定用于任何其他用途。
实验操作小课堂
常见问题解答
查看更多
联系我们获取更多批次信息
资源下载
产品操作手册

产品介绍

生物活性
产品描述
Anti-Peroxiredoxin 5 Antibody (6B923) is a Rabbit antibody targeting Peroxiredoxin 5. Anti-Peroxiredoxin 5 Antibody (6B923) can be used in WB,ICC/IF,IP.
别名PrxV, Prdx6, Pmp20, peroxiredoxin 5, AOPP, AOEB166
Ig Type
Rabbit IgG
克隆号
6B923
交叉反应
Human
验证活性
1. PRDX5 was immunoprecipitated using:
-Lane A:0.5 mg Hela Whole Cell Lysate.
-Lane B:0.5 mg Jurkat Whole Cell Lysate.
-Lane C:0.5 mg 293T Whole Cell Lysate.
-2 µL anti-PRDX5 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-PRDX5 rabbit monoclonal antibody, at 1:500 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 22 kDa.
-Observed band size: 22 kDa.
2. Anti-PRDX5 rabbit monoclonal antibody at 1:500 dilution.
-Lane A: Hela Whole Cell Lysate.
-Lane B: 293T Whole Cell Lysate.
-Lane C: Jurkat Whole Cell Lysate.
-Lane D: 293 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:22 kDa.
-Observed band size:15 kDa.
3. Immunofluorescence staining of PRDX5 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human PRDX5 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).
4. Anti-PRDX5 rabbit monoclonal antibody at 1:1000 dilution.
-Lane A: PRDX5 konckout Hela Whole Cell Lysate.
-Lane B: Hela Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.
-Developed using the ECL technique.
-Performed under reducing conditions.
-Predicted band size:22 kDa.
-Observed band size:15 kDa(Validation Experiment)
应用
WB,ICC/IF,IP
推荐剂量
WB: 1:500-1:2000; ICC-IF: 1:20-1:100; IP: 4-8 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human Peroxiredoxin 5 / PRDX5 (rh Peroxiredoxin 5 / PRDX5; TMPY-02208; P30044-2; Met53-Leu214).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景Peroxiredoxin-5, also known as Alu corepressor 1, Antioxidant enzyme B166, Liver tissue 2D-page spot 71B, Peroxisomal antioxidant enzyme, Thioredoxin peroxidase PMP20, Thioredoxin reductase, PRDX5 and ACR1, is cytoplasm protein that belongs to the peroxiredoxin 2 family. Peroxiredoxin-5 / PRDX5 reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Peroxiredoxin-5 / PRDX5 is involved in intracellular redox signaling. The Peroxiredoxins / Prx are a family of 25 kDa peroxidases that can reduce H2O2 using an electron from thioredoxin (Trx) or other substances. The mammalian Peroxiredoxins / Prx family is divided into six groups ( PRDX1，PRDX2, PRDX3, PRDX4, PRDX5, PRDX6 ) on the basis of homology of amino acid sequences. They are located in the cytosol and play a role in the cell signaling system. All six mammalian peroxiredoxins are expressed in the lung. Peroxiredoxins / Prx is overexpressed in breast cancer tissues to a great extent suggesting that Peroxiredoxins / Prx has a proliferative effect and may be related to cancer development or progression.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Human Peroxiredoxin 5 / PRDX5 protein (TMPY-02208)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

计算器

  • 稀释 计算器

关键词

AOEB-166AOEB 166

评论列表

4个月前
5.0
Rating icon 很棒

评论内容

233
Related Tags: buy Anti-Peroxiredoxin 5 Antibody (6B923) | purchase Anti-Peroxiredoxin 5 Antibody (6B923) | Anti-Peroxiredoxin 5 Antibody (6B923) cost | order Anti-Peroxiredoxin 5 Antibody (6B923)