Anti-Peroxiredoxin 3 Polyclonal Antibody is a Rabbit antibody targeting Peroxiredoxin 3. Anti-Peroxiredoxin 3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Dog,Horse,Rabbit)

1. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Peroxiredoxin 3) Polyclonal Antibody, Unconjugated (TMAB-10198) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Peroxiredoxin 3) Polyclonal Antibody, Unconjugated (TMAB-10198) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Sample:
Lane 1: Mouse Kidney tissue lysates
Lane 2: Mouse Liver tissue lysates
Lane 3: Mouse Lung tissue lysates
Lane 4: Rat Liver tissue lysates
Lane 5: Rat Lung tissue lysates
Lane 6: Rat Stomach tissue lysates
Lane 7: Human 293T cell lysates
Lane 8: Human A431 cell lysates
Lane 9: Human HepG2 cell lysates
Lane 10: Human HeLa cell lysates
Lane 11: Human A549 cell lysates
Primary: Anti-Peroxiredoxin 3 (TMAB-10198) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 25 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human PRDX3

Human

PRDX3

Thioredoxin-dependent peroxide reductase, mitochondrial

Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical-generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.