Anti-Perilipin A Polyclonal Antibody is a Rabbit antibody targeting Perilipin A. Anti-Perilipin A Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Sheep)

1. Sample: Parotid gland (Mouse) Lysate at 40 μg
Primary: Anti-Perilipin A (TMAB-01369) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
2. Sample: Thymus (Mouse) Lysate at 40 μg
Primary: Anti-Perilipin A (TMAB-01369) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
3. Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Perilipin A) Polyclonal Antibody, Unconjugated (TMAB-01369) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 min and DAB staining.
4. Sample:
MCF-7 Cell (Human) Lysate at 30 μg
Primary: Anti-Perilipin A at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Perilipin-1

Human

PLIN1

Perilipin

Metabolism of lipids and lipoproteins,Lipoprotein metabolism,Lipid Droplet Protein,Lipid metabolism,Lipoprotein metabolism,Heart disease,Lipid metabolism

Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness.