Anti-Perilipin A Polyclonal Antibody is a Rabbit antibody targeting Perilipin A. Anti-Perilipin A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Sheep)

1. Sample: Parotid gland (Mouse) Lysate at 40 μg
Primary: Anti-Perilipin A (TMAB-01369) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
2. Sample: Thymus (Mouse) Lysate at 40 μg
Primary: Anti-Perilipin A (TMAB-01369) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa
3. Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Perilipin A) Polyclonal Antibody, Unconjugated (TMAB-01369) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 min and DAB staining.
4. Sample:
MCF-7 Cell (Human) Lysate at 30 μg
Primary: Anti-Perilipin A at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Perilipin-1

Human

Metabolism of lipids and lipoproteins,Lipoprotein metabolism,Lipid Droplet Protein,Lipid metabolism,Lipoprotein metabolism,Heart disease,Lipid metabolism