Anti-PEPT1 Polyclonal Antibody is a Rabbit antibody targeting PEPT1. Anti-PEPT1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat

1. Sample:
Lane 1: Liver (Mouse) Lysate at 40 μg
Lane 2: Liver (Rat) Lysate at 40 μg
Lane 3: Panc-1 (Human) Cell Lysate at 30 μg
Primary: Anti-SLC15A1 (TMAB-01367) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kDa
Observed band size: 83 kDa
2. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PEPT1 Polyclonal Antibody, Unconjugated (TMAB-01367) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PEPT1 Polyclonal Antibody, Unconjugated (TMAB-01367) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PEPT1 Polyclonal Antibody, Unconjugated (TMAB-01367) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PEPT1 Polyclonal Antibody, Unconjugated (TMAB-01367) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PEPT1 Polyclonal Antibody, Unconjugated (TMAB-01367) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PEPT1 Polyclonal Antibody, Unconjugated (TMAB-01367) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

WB: 1:500-1000; IHC-P: 1:50-200; IHC-Fr: 1:400-800; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human PEPT1

Human

SLC15A1

Solute carrier family 15 member 1

Amino acid metabolism,Amino Acids,Channels

Proton-coupled intake of oligopeptides of 2 to 4 amino acids with a preference for dipeptides. May constitute a major route for the absorption of protein digestion end-products.