Anti-PDGF-BB Polyclonal Antibody 2 is a Rabbit antibody targeting PDGF-BB. Anti-PDGF-BB Polyclonal Antibody 2 can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PDGFBB) Polyclonal Antibody, Unconjugated (TMAB-01353) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-PDGF-BB Polyclonal Antibody, Unconjugated (TMAB-01353) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-PDGF-BB Polyclonal Antibody, Unconjugated (TMAB-01353) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. Sample:
Lane 1: Huvec (Human) Cell Lysate at 30 μg
Lane 2: A431 (Human) Cell Lysate at 30 μg
Lane 3: MCF-7 (Human) Cell Lysate at 30 μg
Primary:
Anti-PDGFBB (TMAB-01353) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27/22/14 kDa
Observed band size: 30 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human PDGF-B

Human

PDGFB

Platelet-derived growth factor subunit B

Response to hypoxia,PDGF,Inflammatory mediators,Serum Proteins,Angiogenesis and vasculogenesis,Hypoxia,Heart disease,PDGF

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.